Mouse Carbohydrate sulfotransferase 5 ELISA Kit | CHST5 elisa kit
Mouse Carbohydrate sulfotransferase 5 (CHST5) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHST5 in Mouse serum, plasma. An antibody specific for CHST5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHST5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHST5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHST5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
Uniprot Description
CHST6: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N- acetyllactosamine structures. Defects in CHST6 are the cause of macular corneal dystrophy (MCD). MCD is an autosomal recessive disease characterized by corneal opacities. Onset occurs in the first decade, usually between ages 5 and 9. The disorder is progressive. Minute, gray, punctate opacities develop. Corneal sensitivity is usually reduced. Painful attacks with photophobia, foreign body sensations, and recurrent erosions occur in most patients. There are different types of MCD: MCD type I, in which there is a virtual absence of sulfated keratan sulfate (KS) in the serum and cornea, as determined by KS-specific antibodies; and MCD type II, in which the normal sulfated KS-antibody response is present in cornea and serum. MCD type I patients usually have a homozygous missense mutation, while MCD type II patients show a large deletion and replacement in the upstream region of CHST6. The only missense mutation for type II is Cys-50, which is heterozygous with a replacement in the upstream region on the other allele of CHST6. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Protein type: EC 2.8.2.-; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral; Transferase
Chromosomal Location of Human Ortholog: 8|8 E1
Cellular Component: Golgi apparatus; Golgi membrane; integral component of membrane; membrane
Molecular Function: N-acetylglucosamine 6-O-sulfotransferase activity; sulfotransferase activity; transferase activity
Biological Process: carbohydrate metabolic process; keratan sulfate biosynthetic process; N-acetylglucosamine metabolic process; sulfur compound metabolic process
Research Articles on CHST5
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Product Notes
The Mouse CHST5 chst5 (Catalog #AAA280566) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280566 ELISA Kit recognizes Mouse CHST5. It is sometimes possible for the material contained within the vial of "Carbohydrate sulfotransferase 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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