Human Keratin, type II cuticular Hb6 (KRT86) ELISA Kit | KRT86 elisa kit

Human Keratin, type II cuticular Hb6 (KRT86) ELISA Kit

Cat. Num. AAA2802164

• Gene Names: KRT86; HB6; Hb1; MNX; hHb6; KRTHB1; KRTHB6
• Reactivity: Human
• Synonyms: Keratin; type II cuticular Hb6 (KRT86); Human Keratin; type II cuticular Hb6 (KRT86) ELISA Kit; FLJ25176; HB6; Hb1; KRTHB1; KRTHB6; MNX; hHb6; hard keratin; type II; 6|keratin protein HB6|keratin; hair; basic; 6 (monilethrix); KRT86 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KRT86. No significant cross-reactivity or interference between Human KRT86 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate KRT86 in samples. An antibody specific for KRT86 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT86 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT86 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT86 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

• NCBI GI #: 14318422
• NCBI GeneID: 3892
• NCBI Accession #: NP_002275.1
• NCBI GenBank Nucleotide #: NM_002284.3
• UniProt Accession #: O43790; P78387
• Molecular Weight: 53,501 Da
• NCBI Official Full Name: keratin, type II cuticular Hb6
• NCBI Official Synonym Full Names: keratin 86
• NCBI Official Symbol: KRT86
• NCBI Official Synonym Symbols: HB6; Hb1; MNX; hHb6; KRTHB1; KRTHB6
• NCBI Protein Information: keratin, type II cuticular Hb6; K86; keratin-86; hair keratin K2.11; keratin protein HB6; type-II keratin Kb26; hard keratin, type II, 6; type II hair keratin Hb6; keratin, hair, basic, 6 (monilethrix)
• UniProt Protein Name: Keratin, type II cuticular Hb6
• Protein Family: Keratin
• UniProt Gene Name: KRT86
• UniProt Synonym Gene Names: KRTHB6; K86
• UniProt Entry Name: KRT86_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB1 and KRTHB3, is found primarily in the hair cortex. Mutations in this gene and KRTHB1 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq, Jul 2008]

Uniprot Description

Subunit structure: Heterotetramer of two type I and two type II keratins.

Tissue specificity: Synthesis begins slightly higher in the hair shaft than HB1 and HB3 and continues much farther up, ending in the keratogeneous zone. Ref.3

Involvement in disease: Monilethrix (MLTRX) [MIM:158000]: Autosomal dominant hair disorder characterized clinically by alopecia and follicular papules. Affected hairs have uniform elliptical nodes of normal thickness and intermittent constrictions, internodes at which the hair easily breaks. Usually only the scalp is involved, but in severe forms, the secondary sexual hair, eyebrows, eyelashes, and nails may also be affected.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.5 Ref.6 Ref.7

Miscellaneous: There are two types of hair/microfibrillar keratin, I (acidic) and II (neutral to basic).

Sequence similarities: Belongs to the intermediate filament family.