General Lipoxin A4 ELISA Kit | LXA4 elisa kit

Lipoxin A4 ELISA Kit

Cat. Num. AAA762533

• Gene Names: FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II
• Reactivity: General
• Synonyms: Lipoxin A4; Lipoxin A4 ELISA Kit; LXA4 (Lipoxin A4); LXA4 elisa kit

• Reactivity: General
• Specificity: This assay has high sensitivity and excellent specificity for detection of LXA4. No significant cross-reactivity or interference between LXA4 and analogues was observed.
• Sequence Length: 351

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LXA4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LXA4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LXA4 elisa kit

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with LXA4. During the reaction, LXA4 in the sample or standard competes with a fixed amount of LXA4 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to LXA4. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LXA4 in the samples is then determined by comparing the OD. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1916076
• NCBI GeneID: 2358
• Molecular Weight: 38,964 Da
• NCBI Official Full Name: lipoxin A4 receptor
• NCBI Official Synonym Full Names: formyl peptide receptor 2
• NCBI Official Symbol: FPR2
• NCBI Official Synonym Symbols: ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II
• NCBI Protein Information: N-formyl peptide receptor 2
• UniProt Protein Name: N-formyl peptide receptor 2
• UniProt Gene Name: FPR2
• UniProt Synonym Gene Names: FPRH1; FPRL1; LXA4R; FMLP-R-I; LXA4 receptor
• UniProt Entry Name: FPR2_HUMAN

Uniprot Description

FPR2: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). Belongs to the G-protein coupled receptor 1 family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; N-formyl peptide receptor activity

Biological Process: cell adhesion; cell motility; chemotaxis; G-protein coupled receptor protein signaling pathway; inflammatory response