Mouse Latent-transforming growth factor beta-binding protein 2 (LTBP2) ELISA Kit | LTBP2 elisa kit

Mouse Latent-transforming growth factor beta-binding protein 2 (LTBP2) ELISA Kit

Cat. Num. AAA2802150

• Gene Names: Ltbp2; AW208642
• Reactivity: Mouse
• Synonyms: Latent-transforming growth factor beta-binding protein 2 (LTBP2); Mouse Latent-transforming growth factor beta-binding protein 2 (LTBP2) ELISA Kit; C14orf141; GLC3D; LTBP3; MSTP031; LTBP2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse LTBP2. No significant cross-reactivity or interference between Mouse LTBP2 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate LTBP2 in samples. An antibody specific for LTBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLTBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LTBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LTBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

Related Product Information for LTBP2 elisa kit

LTBP2 belongs to the family of latent transforming growth factor (TGF)-beta binding proteins (LTBP), which are extracellular matrix proteins with multi-domain structure. This protein is the largest member of the LTBP family possessing unique regions and with most similarity to the fibrillins. It has thus been suggested that it may have multiple functions: as a member of the TGF-beta latent complex, as a structural component of microfibrils, and a role in cell adhesion. LTBP2 has approximately 45% amino acid identity to LTBP1 and 25% identity to fibrillin-1 (FBN1) but unlike other LTBP family members does not bind to latent forms of TGFB. The C-terminal region of LTBP2 competes with LTBP1 for specific binding to the N-terminal region of FBN1, but not FBN5, in microfibrils.

NCBI and Uniprot Product Information

• NCBI GI #: 158341636
• NCBI GeneID: 16997
• NCBI Accession #: NP_038617.3
• NCBI GenBank Nucleotide #: NM_013589.3
• UniProt Accession #: O08999; Q8C6W9
• Molecular Weight: 195,829 Da
• NCBI Official Full Name: latent-transforming growth factor beta-binding protein 2
• NCBI Official Synonym Full Names: latent transforming growth factor beta binding protein 2
• NCBI Official Symbol: Ltbp2
• NCBI Official Synonym Symbols: AW208642
• NCBI Protein Information: latent-transforming growth factor beta-binding protein 2
• UniProt Protein Name: Latent-transforming growth factor beta-binding protein 2
• Protein Family: Latent-transforming growth factor beta-binding protein
• UniProt Gene Name: Ltbp2
• UniProt Synonym Gene Names: LTBP-2
• UniProt Entry Name: LTBP2_MOUSE

Uniprot Description

LTBP2: May play an integral structural role in elastic-fiber architectural organization and/or assembly. Defects in LTBP2 are the cause of primary congenital glaucoma type 3D (GLC3D). An autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. Defects in LTBP2 are the cause of microspherophakia (MCSPH). It is a rare disease characterized by smaller and more spherical lenses than normal bilaterally, an increased anteroposterior thickness of the lens and highly myopic eyes. Lens dislocation or subluxation may occur, leading to defective accommodation. Defects in LTBP2 are the cause of Weill-Marchesani syndrome 3 (WMS3). A rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and lens abnormalities. Belongs to the LTBP family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; growth factor binding; calcium ion binding