Human Derlin-1 (DERL1) ELISA Kit | DERL1 elisa kit

Human Derlin-1 (DERL1) ELISA Kit

Cat. Num. AAA2800158

• Gene Names: DERL1; DER1; DER-1; derlin-1
• Reactivity: Human
• Synonyms: Derlin-1 (DERL1); Human Derlin-1 (DERL1) ELISA Kit; DER-1; DER1; FLJ13784; FLJ42092; MGC3067; PRO2577; derlin-1; DERL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human DERL1. No significant cross-reactivity or interference between Human DERL1 and analogues was observed.
• Sequence Length: 231

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DERL1 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate DERL1 in samples. An antibody specific for DERL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DERL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DERL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DERL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Derlin-1 is a widely expressed protein, with strong signals obtained by immunoblotting from liver, spleen, pancreas, lung, thymus, and ovary. Despite the presence of Derlin-1 sequences in brain cDNA libraries, immunoreactivity was not detected in brain.Derlin-1 as an ER membrane protein essential for US11-mediated dislocation of the major histocompatibility complex (MHC) class I heavy chain from the ER to the cytosol, and showed that Derlin-1 mediates the degradation of 2 different type 1 membrane proteins, class I MHC heavy chain and US2.The central component of the complex, Derlin-1, was found to associate with different substrates as they move through the membrane. Inactivation of Derlin-1 in C. elegans caused ER stress.

NCBI and Uniprot Product Information

• NCBI GI #: 197927278
• NCBI GeneID: 79139
• NCBI Accession #: NP_001128143.1
• NCBI GenBank Nucleotide #: NM_001134671.2
• UniProt Accession #: Q9BUN8; B3KW41; E9PH19
• Molecular Weight: 26,423 Da
• NCBI Official Full Name: derlin-1 isoform b
• NCBI Official Synonym Full Names: derlin 1
• NCBI Official Symbol: DERL1
• NCBI Official Synonym Symbols: DER1; DER-1; derlin-1
• NCBI Protein Information: derlin-1
• UniProt Protein Name: Derlin-1
• Protein Family: Probable derlin
• UniProt Gene Name: DERL1
• UniProt Synonym Gene Names: DERtrin-1

NCBI Description

The protein encoded by this gene is a member of the derlin family. Members of this family participate in the ER-associated degradation response and retrotranslocate misfolded or unfolded proteins from the ER lumen to the cytosol for proteasomal degradation. This protein recognizes substrate in the ER and works in a complex to retrotranslocate it across the ER membrane into the cytosol. This protein may select cystic fibrosis transmembrane conductance regulator protein (CFTR) for degradation as well as unfolded proteins in Alzheimer's disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the misfolded protein (PubMed:15215856). Also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation (PubMed:26565908).