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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUDT9 protein (Right) or empty vector (Left) detected with NUDT9 antibody)

Mouse NUDT9 Monoclonal Antibody | anti-NUDT9 antibody

NUDT9 antibody

Gene Names
NUDT9; NUDT10
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
NUDT9 antibody was purified by affinity chromatography.
Synonyms
NUDT9; Monoclonal Antibody; NUDT9 antibody; Monoclonal NUDT9; Anti-NUDT9; MGC3037; NUDT10; Nucleoside Diphosphate Linked Moiety X-Type Motif 9; Nudix 9; NUDT 9; NUDT-9; anti-NUDT9 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7A12
Purity/Purification
NUDT9 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
350
Applicable Applications for anti-NUDT9 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
Human ADP-ribose pyrophosphatase NUDT9 belongs to a superfamily of Nudix hydrolases that catabolize potentially toxic compounds in the cell. NUDT9 alpha protein is targeted highly specifically to mitochondria, whereas the predicted protein of the NUDT9 beta transcript, which is missing this sequence, exhibits no clear subcellular localization. Investigation of the physical and enzymatic properties of NUDT9 indicates that it is functional as a monomer, optimally active at near neutral pH, and that it requires divalent metal ions and an intact Nudix motif for enzymatic activity.
Cross-Reactivity
Human
Immunogen
NUDT9 antibody was raised in mouse using a full length recombinant protein of human NUDT9 (NP_076952) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUDT9 protein (Right) or empty vector (Left) detected with NUDT9 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUDT9 protein (Right) or empty vector (Left) detected with NUDT9 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NUDT9 protein in paraffin embedded Human endometrium tissue using NUDT9 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of NUDT9 protein in paraffin embedded Human endometrium tissue using NUDT9 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUDT9 protein using NUDT9 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NUDT9 protein using NUDT9 antibody)
Related Product Information for anti-NUDT9 antibody
Mouse monoclonal NUDT9 antibody
Product Categories/Family for anti-NUDT9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
33,776 Da
NCBI Official Full Name
NUDT9
NCBI Official Synonym Full Names
nudix (nucleoside diphosphate linked moiety X)-type motif 9
NCBI Official Symbol
NUDT9
NCBI Official Synonym Symbols
NUDT10
NCBI Protein Information
ADP-ribose pyrophosphatase, mitochondrial
UniProt Protein Name
ADP-ribose pyrophosphatase, mitochondrial
Protein Family
UniProt Gene Name
NUDT9
UniProt Synonym Gene Names
NUDT10; ADPR-PPase; Nudix motif 9
UniProt Entry Name
NUDT9_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Nudix hydrolase family. Nudix boxes are found in a family of diverse enzymes that catalyze the hydrolysis of nucleoside diphosphate derivatives. This enzyme is an ADP-ribose pyrophosphatase that catalyzes the hydrolysis of ADP-ribose to AMP and ribose-5-P. It requires divalent metal ions and an intact Nudix motif for enzymatic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

NUDT9: Hydrolyzes ADP-ribose (ADPR) to AMP and ribose 5'- phosphate. Belongs to the Nudix hydrolase family. NudF subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Mitochondrial; Hydrolase; EC 3.6.1.13

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: mitochondrial matrix; intracellular

Molecular Function: ADP-ribose diphosphatase activity; ADP-sugar diphosphatase activity; adenosine-diphosphatase activity

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; ADP catabolic process; IDP catabolic process

Research Articles on NUDT9

Similar Products

Product Notes

The NUDT9 nudt9 (Catalog #AAA838614) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NUDT9 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the NUDT9 nudt9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUDT9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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