Mouse Interleukin 5 (IL5) ELISA Kit | IL5 elisa kit
Mouse Interleukin 5 (IL5) Mini Samples ELISA Kit
Gene Names
Il5; Il-5
Reactivity
Mouse
Applications
ELISA
Synonyms
Interleukin 5 (IL5); Mouse Interleukin 5 (IL5) Mini Samples ELISA Kit; Interleukin 5; E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor; Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor; IL5 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 5 (IL5).
No significant cross-reactivity or interference between Mini Samples Interleukin 5 (IL5) and analogues was observed.
No significant cross-reactivity or interference between Mini Samples Interleukin 5 (IL5) and analogues was observed.
Sequence Length
133
Applicable Applications for IL5 elisa kit
ELISA (EIA)
Assay Type
Double-antibody Sandwich
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 5 (IL5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 5 (IL5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 5 (IL5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 5 (IL5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 5 (IL5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 5 (IL5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Interleukin 5 (IL5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-5
NCBI Official Synonym Full Names
interleukin 5
NCBI Official Symbol
Il5
NCBI Official Synonym Symbols
Il-5
NCBI Protein Information
interleukin-5
UniProt Protein Name
Interleukin-5
Protein Family
UniProt Gene Name
Il5
UniProt Synonym Gene Names
Il-5; IL-5; BCGF-II; TRF
UniProt Entry Name
IL5_MOUSE
Uniprot Description
IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.
Protein type: Secreted, signal peptide; Cytokine; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-5 receptor binding; growth factor activity; cytokine activity
Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; positive regulation of eosinophil differentiation
Research Articles on IL5
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Product Notes
The Mouse IL5 il5 (Catalog #AAA2708232) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708232 ELISA Kit recognizes Mouse IL5. AAA Biotech's Interleukin 5 (IL5) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the IL5 il5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 5 (IL5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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