Rabbit anti-Human, Mouse PROX-1 Polyclonal Antibody | anti-PROX1 antibody

PROX-1 (S514) (PROX1, Prospero homeobox protein 1, Homeobox prospero-like protein PROX1)

Cat. Num. AAA644835

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PROX-1; Polyclonal Antibody; PROX-1 (S514) (PROX1; Prospero homeobox protein 1; Homeobox prospero-like protein PROX1); Anti -PROX-1 (S514) (PROX1; anti-PROX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PROX1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: PROX-1-S514 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 500-530 from the region of human PROX.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) using MBS644835. PROX-1 (arrow) was detected using MBS644835.)

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using MBS644835. PROX-1 (arrow) was detected using MBS644835.)

Testing Data

(All lanes: 044084 (1:2000) Lane 1: HepG2 whole cell lysate (20ug/lane) Lane 2: SH-SY5Y whole cell lysate (20ug/lane) Lane 3: SW620 whole cell lysate (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 83kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS644835 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS644835 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PROX1 antibody

Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Product Categories/Family for anti-PROX1 antibody

Antibodies; Abs to Transcription Factors; Zinc; Ring; Homeobox

NCBI and Uniprot Product Information

• NCBI GI #: 56785422
• NCBI GeneID: 395802
• NCBI Accession #: NP_001005616.1
• NCBI GenBank Nucleotide #: NM_001005616.1
• UniProt Accession #: Q91018
• Molecular Weight: 83,141 Da
• NCBI Official Full Name: prospero homeobox protein 1
• NCBI Official Synonym Full Names: prospero homeobox 1
• NCBI Official Symbol: PROX1
• NCBI Protein Information: prospero homeobox protein 1; PROX-1; prospero-related homeobox 1; homeobox prospero-like protein PROX1
• UniProt Protein Name: Prospero homeobox protein 1
• Protein Family: Prospero homeobox protein
• UniProt Gene Name: PROX1
• UniProt Synonym Gene Names: PROX-1
• UniProt Entry Name: PROX1_CHICK

Uniprot Description

Function: May be essential in the development and function of the eye.

Subcellular location: Nucleus

Probable.

Tissue specificity: Expressed most actively in the developing lens and midgut and at lower levels in the developing brain, heart, muscle and retina.

Developmental stage: First detected at stage 14 in the early lens placode. At later stages of development, it was observed throughout the lens, but it appeared more abundant around the bow region of the equator than in the anterior epithelium or the fibers. In the retina, expression was detected mainly in the inner nuclear layer during later stages of histogenesis.

Sequence similarities: Belongs to the Prospero homeobox family.Contains 1 Prospero-type homeobox DNA-binding domain.