Human Claudin 3 (CLDN3) ELISA Kit | CLDN3 elisa kit

Human Claudin 3 (CLDN3) High Sensitive ELISA Kit

Cat. Num. AAA2708114

• Gene Names: CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
• Reactivity: Human
• Applications: ELISA
• Synonyms: Claudin 3 (CLDN3); Human Claudin 3 (CLDN3) High Sensitive ELISA Kit; Claudin 3; C7orf1; CPE-R2; CPETR2; HRVP1; RVP1; Clostridium Perfringens Enterotoxin Receptor 2; Ventral Prostate.1-Like Protein; CPE-Receptor 2; CLDN3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Claudin 3 (CLDN3).; No significant cross-reactivity or interference between High Sensitive Claudin 3 (CLDN3) and analogues was observed.
• Sequence Length: 220

• Applicable Applications for CLDN3 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 78-5000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 35pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Claudin 3 (CLDN3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Claudin 3 (CLDN3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Claudin 3 (CLDN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Claudin 3 (CLDN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Claudin 3 (CLDN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Claudin 3 (CLDN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 4502875
• NCBI GeneID: 1365
• NCBI Accession #: NP_001297.1
• NCBI GenBank Nucleotide #: NP_001297.1
• UniProt Accession #: O15551
• NCBI Official Full Name: claudin-3
• NCBI Official Synonym Full Names: claudin 3
• NCBI Official Symbol: CLDN3
• NCBI Official Synonym Symbols: RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
• NCBI Protein Information: claudin-3
• UniProt Protein Name: Claudin-3
• Protein Family: Claudin
• UniProt Gene Name: CLDN3
• UniProt Synonym Gene Names: C7orf1; CPETR2; CPE-R 2; CPE-receptor 2; hRVP1
• UniProt Entry Name: CLD3_HUMAN

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]

Uniprot Description

Claudin-3: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the claudin family.

Protein type: Cytoskeletal; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: tight junction; integral to plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: identical protein binding; transmembrane receptor activity; structural molecule activity

Biological Process: response to hypoxia; signal transduction; calcium-independent cell-cell adhesion