Cattle Reactive Oxygen Species Modulator 1 (ROMO1) ELISA Kit | ROMO1 elisa kit

Cattle Reactive Oxygen Species Modulator 1 (ROMO1) ELISA Kit

Cat. Num. AAA2707814

• Reactivity: Cattle
• Synonyms: Reactive Oxygen Species Modulator 1 (ROMO1); Cattle Reactive Oxygen Species Modulator 1 (ROMO1) ELISA Kit; C20orf52; MTGMP; Glyrichin; Mitochondrial Targeting GXXXG Protein; Epididymis tissue protein Li 175; Protein MGR2 homolog; ROMO1 elisa kit

• Reactivity: Cattle
• Specificity: This assay has high sensitivity and excellent specificity for detection of Reactive Oxygen Species Modulator 1 (ROMO1).; No significant cross-reactivity or interference between Reactive Oxygen Species Modulator 1 (ROMO1) and analogues was observed.
• Sequence Length: 79

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.119ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Reactive Oxygen Species Modulator 1 (ROMO1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Reactive Oxygen Species Modulator 1 (ROMO1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ROMO1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reactive Oxygen Species Modulator 1 (ROMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Reactive Oxygen Species Modulator 1 (ROMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Reactive Oxygen Species Modulator 1 (ROMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reactive Oxygen Species Modulator 1 (ROMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18152785
• NCBI GenBank Nucleotide #: NP_542786.1
• UniProt Accession #: P60602
• NCBI Official Full Name: reactive oxygen species modulator 1
• UniProt Protein Name: Reactive oxygen species modulator 1
• Protein Family: Reactive oxygen species modulator
• UniProt Gene Name: ROMO1
• UniProt Synonym Gene Names: C20orf52; ROS modulator 1; MTGM
• UniProt Entry Name: ROMO1_HUMAN

Uniprot Description

ROMO1: Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation. Belongs to the MGR2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Biological Process: positive regulation of cell proliferation; defense response to bacterium; replicative cell aging