Human Fatty Acid Amide Hydrolase (FAAH) ELISA Kit | FAAH elisa kit

Human Fatty Acid Amide Hydrolase (FAAH) ELISA Kit

Cat. Num. AAA2707213

• Gene Names: FAAH; PSAB; FAAH-1
• Reactivity: Human
• Synonyms: Fatty Acid Amide Hydrolase (FAAH); Human Fatty Acid Amide Hydrolase (FAAH) ELISA Kit; FAAH-1; Anandamide amidohydrolase 1; Oleamide hydrolase 1; FAAH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Amide Hydrolase (FAAH).; No significant cross-reactivity or interference between Fatty Acid Amide Hydrolase (FAAH) and analogues was observed.
• Sequence Length: 579

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.60ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Amide Hydrolase (FAAH) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Amide Hydrolase (FAAH) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FAAH elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Amide Hydrolase (FAAH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Amide Hydrolase (FAAH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Amide Hydrolase (FAAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Amide Hydrolase (FAAH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4249693
• NCBI GeneID: 2166
• Molecular Weight: 63,066 Da
• NCBI Official Full Name: fatty acid amide hydrolase
• NCBI Official Synonym Full Names: fatty acid amide hydrolase
• NCBI Official Symbol: FAAH
• NCBI Official Synonym Symbols: PSAB; FAAH-1
• NCBI Protein Information: fatty-acid amide hydrolase 1
• UniProt Protein Name: Fatty-acid amide hydrolase 1
• Protein Family: Fatty-acid amide hydrolase
• UniProt Gene Name: FAAH
• UniProt Synonym Gene Names: FAAH1

NCBI Description

This gene encodes a protein that is responsible for the hydrolysis of a number of primary and secondary fatty acid amides, including the neuromodulatory compounds anandamide and oleamide. [provided by RefSeq, Jul 2008]

Uniprot Description

Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.