Human Serine/Threonine Kinase 24 (STK24) ELISA Kit | STK24 elisa kit

Human Serine/Threonine Kinase 24 (STK24) ELISA Kit

Cat. Num. AAA2707183

• Gene Names: STK24; MST3; STK3; MST3B; STE20; HEL-S-95
• Reactivity: Human
• Synonyms: Serine/Threonine Kinase 24 (STK24); Human Serine/Threonine Kinase 24 (STK24) ELISA Kit; MST3; MST3B; STK3; STE20; STE20-Like Kinase 3; Sterile 20-Like Kinase 3; Mammalian STE20-like protein kinase 3; STK24 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Serine/Threonine Kinase 24 (STK24).; No significant cross-reactivity or interference between Serine/Threonine Kinase 24 (STK24) and analogues was observed.
• Sequence Length: 431

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.59ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 24 (STK24) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 24 (STK24) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STK24 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 24 (STK24). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 24 (STK24). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Threonine Kinase 24 (STK24), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/Threonine Kinase 24 (STK24) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13626607
• NCBI GeneID: 8428
• UniProt Accession #: Q9Y6E0; O14840; Q5JV92
• Molecular Weight: 47,913 Da
• NCBI Official Full Name: Serine/threonine-protein kinase 24
• NCBI Official Synonym Full Names: serine/threonine kinase 24
• NCBI Official Symbol: STK24
• NCBI Official Synonym Symbols: MST3; STK3; MST3B; STE20; HEL-S-95
• NCBI Protein Information: serine/threonine-protein kinase 24
• UniProt Protein Name: Serine/threonine-protein kinase 24
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK24
• UniProt Synonym Gene Names: MST3; STK3; MST-3; MST3/N; MST3/C

NCBI Description

This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Uniprot Description

Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed:27807006). Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.