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Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, A375 whole cell lysate, HepG2 whole cell lysateAll lanes: STK24 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50,48 KDaObserved band size: 50 KDa)

Rabbit anti-Human Serine/threonine-protein kinase 24 Polyclonal Antibody | anti-STK24 antibody

Rabbit anti-human Serine/threonine-protein kinase 24 polyclonal Antibody

Gene Names
STK24; MST3; STK3; MST3B; STE20; HEL-S-95
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Serine/threonine-protein kinase 24; Polyclonal Antibody; Rabbit anti-human Serine/threonine-protein kinase 24 polyclonal Antibody; Mammalian STE20-like protein kinase 3 Mammalian STE20-like protein kinase 3 N-terminal Mammalian STE20-like protein kinase 3 C-terminal STK24 MST3; STK3; anti-STK24 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
431
Applicable Applications for anti-STK24 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000
Immunogen
Recombinant human Serine/threonine-protein kinase 24 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21400
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, A375 whole cell lysate, HepG2 whole cell lysateAll lanes: STK24 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50,48 KDaObserved band size: 50 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, A375 whole cell lysate, HepG2 whole cell lysateAll lanes: STK24 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50,48 KDaObserved band size: 50 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7005042 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7005042 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescence staining of Hela cells with MBS7005042 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Immunohistochemistry (IHC) (Immunofluorescence staining of Hela cells with MBS7005042 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-STK24 antibody
Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,913 Da
NCBI Official Full Name
serine/threonine-protein kinase 24 isoform b
NCBI Official Synonym Full Names
serine/threonine kinase 24
NCBI Official Symbol
STK24
NCBI Official Synonym Symbols
MST3; STK3; MST3B; STE20; HEL-S-95
NCBI Protein Information
serine/threonine-protein kinase 24
UniProt Protein Name
Serine/threonine-protein kinase 24
UniProt Gene Name
STK24
UniProt Synonym Gene Names
MST3; STK3; MST-3; MST3/N; MST3/C
UniProt Entry Name
STK24_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Uniprot Description

MST3: a protein kinase of the STE20 family. Activated by caspase-mediated cleavage

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE20 family; YSK subfamily

Chromosomal Location of Human Ortholog: 13q31.2-q32.3

Cellular Component: cytoplasm; cytosol; membrane; nucleolus; nucleoplasm; nucleus

Molecular Function: ATP binding; metal ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; Rac GTPase binding

Biological Process: actin cytoskeleton organization and biogenesis; activation of protein kinase activity; apoptosis; cell migration; cell structure disassembly during apoptosis; induction of apoptosis by oxidative stress; negative regulation of cell migration; programmed cell death; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of axon regeneration; regulation of MAPKKK cascade; regulation of mitotic cell cycle; response to hydrogen peroxide; Rho protein signal transduction; signal transduction; stress-activated protein kinase signaling pathway

Research Articles on STK24

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Product Notes

The STK24 stk24 (Catalog #AAA7005042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Serine/threonine-protein kinase 24 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Serine/threonine-protein kinase 24 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the STK24 stk24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serine/threonine-protein kinase 24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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