Human Abl Interactor 1 (ABI1) ELISA Kit | ABI1 elisa kit

Human Abl Interactor 1 (ABI1) ELISA Kit

Cat. Num. AAA2706749

• Gene Names: Abi1; E3B1; NAP1; abi-1; Ssh3bp1
• Reactivity: Human
• Synonyms: Abl Interactor 1 (ABI1); Human Abl Interactor 1 (ABI1) ELISA Kit; ABI-1; E3B1; NAP1BP; SSH3BP1; Spectrin SH3 Domain Binding Protein 1; Abelson interactor 1; Abl-binding protein 4; Nap1-binding protein; Eps8 SH3 domain-binding protein; ABI1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Abl Interactor 1 (ABI1).; No significant cross-reactivity or interference between Abl Interactor 1 (ABI1) and analogues was observed.
• Sequence Length: 388

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Abl Interactor 1 (ABI1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Abl Interactor 1 (ABI1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABI1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Abl Interactor 1 (ABI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Abl Interactor 1 (ABI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Abl Interactor 1 (ABI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Abl Interactor 1 (ABI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 116089341
• NCBI GeneID: 11308
• NCBI Accession #: NP_001070658.1
• NCBI GenBank Nucleotide #: NM_001077190.3
• UniProt Accession #: Q8CBW3; Q3U8V0; Q60747; Q91ZM5; Q923I9; Q99KH4
• Molecular Weight: 42,548 Da
• NCBI Official Full Name: abl interactor 1 isoform 1
• NCBI Official Synonym Full Names: abl-interactor 1
• NCBI Official Symbol: Abi1
• NCBI Official Synonym Symbols: E3B1; NAP1; abi-1; Ssh3bp1
• NCBI Protein Information: abl interactor 1
• UniProt Protein Name: Abl interactor 1
• Protein Family: Abl interactor
• UniProt Gene Name: Abi1
• UniProt Synonym Gene Names: Abi-1; Eps8-binding protein

Uniprot Description

May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. In vitro, at least isoform 2 and isoform 4 suppress the transforming activity of Abelson murine leukemia virus (v-Abl) after overexpression in fibroblasts. May play a role in regulation EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.