Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LDLR chaperone MESD (MESDC2) Standard Curve.)
Human LDLR Chaperone MESD (MESDC2) ELISA Kit | MESDC2 elisa kit
Human LDLR Chaperone MESD (MESDC2) ELISA Kit
Gene Names
MESD; BOCA; MESDC2
Reactivity
Human
Synonyms
LDLR Chaperone MESD (MESDC2); Human LDLR Chaperone MESD (MESDC2) ELISA Kit; MESDC2; BOCA; KIAA0081; MESD; MESDC2 elisa kit
Reactivity
Human
Specificity
Human LDLR chaperone MESD (MESDC2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MESDC2. No significant cross-reactivity or interference between Human MESDC2 and analogues was observed.
Sequence Length
234
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LDLR chaperone MESD (MESDC2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LDLR chaperone MESD (MESDC2) Standard Curve.)
Related Product Information for MESDC2 elisa kit
Description: This Human LDLR chaperone MESD (MESDC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MESDC2 in samples. An antibody specific for MESDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MESDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MESDC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MESDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MESDC2 (Mesoderm Development Candidate 2) is a Protein Coding gene. Among its related pathways are Wnt/ Hedgehog/ Notch. GO annotations related to this gene include low-density lipoprotein particle receptor binding. Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction.
Background: MESDC2 (Mesoderm Development Candidate 2) is a Protein Coding gene. Among its related pathways are Wnt/ Hedgehog/ Notch. GO annotations related to this gene include low-density lipoprotein particle receptor binding. Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
LRP chaperone MESD
NCBI Official Synonym Full Names
mesoderm development LRP chaperone
NCBI Official Symbol
MESD
NCBI Official Synonym Symbols
BOCA; MESDC2
NCBI Protein Information
LRP chaperone MESD
UniProt Protein Name
LDLR chaperone MESD
Protein Family
UniProt Gene Name
MESDC2
UniProt Synonym Gene Names
KIAA0081; MESD
UniProt Entry Name
MESD_HUMAN
Uniprot Description
MESDC2: Chaperone specifically assisting the folding of beta- propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction. Monomer. Interacts with LRP5; the interaction prevents LRP5 from forming aggregates and chaperones LRP6 to the plasma membrane. Interacts with LRP6; the interaction prevents LRP6 from forming aggregates and chaperones LRP6 to the plasma membrane. Belongs to the MESD family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 15q13
Cellular Component: endoplasmic reticulum; plasma membrane
Molecular Function: low-density lipoprotein receptor binding
Biological Process: Wnt receptor signaling pathway; protein folding; mesoderm development
Research Articles on MESDC2
Similar Products
Product Notes
The Human MESDC2 mesdc2 (Catalog #AAA9715312) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715312 ELISA Kit recognizes Human MESDC2. It is sometimes possible for the material contained within the vial of "LDLR Chaperone MESD (MESDC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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