Mouse Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit | LPCAT3 elisa kit

Mouse Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit

Cat. Num. AAA2706163

• Gene Names: LPCAT3; C3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5
• Reactivity: Mouse
• Synonyms: Lysophosphatidylcholine Acyltransferase 3 (LPCAT3); Mouse Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit; MBOAT5; C3F; OACT5; Nessy; LPSAT; Membrane Bound O-AcylTransferase Domain Containing 5; Lysophospholipid Acyltransferase 5; Lysophosphatidylserine acyltransferase; LPCAT3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysophosphatidylcholine Acyltransferase 3 (LPCAT3).; No significant cross-reactivity or interference between Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) and analogues was observed.
• Sequence Length: 407

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LPCAT3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 3 (LPCAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 40850972
• NCBI GeneID: 10162
• UniProt Accession #: Q6P1A2; Q7KZS1; Q92980; Q9BW40; B2RDH0; B7Z3N3
• Molecular Weight: 46,765 Da
• NCBI Official Full Name: Lysophosphatidylcholine acyltransferase 3
• NCBI Official Synonym Full Names: lysophosphatidylcholine acyltransferase 3
• NCBI Official Symbol: LPCAT3
• NCBI Official Synonym Symbols: C3F; LPCAT; LPSAT; OACT5; nessy; MBOAT5; LPLAT 5
• NCBI Protein Information: lysophospholipid acyltransferase 5
• UniProt Protein Name: Lysophospholipid acyltransferase 5
• Protein Family: Lysophospholipid acyltransferase
• UniProt Gene Name: LPCAT3
• UniProt Synonym Gene Names: MBOAT5; OACT5; LPLAT 5; LPCAT; Lyso-PC acyltransferase; Lyso-PC acyltransferase 3; LPSAT; Lyso-PS acyltransferase; O-acyltransferase domain-containing protein 5

Uniprot Description

Acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (LPCAT activity). Catalyzes also the conversion of lysophosphatidylserine (1-acyl-2-hydroxy-sn-glycero-3-phospho-L-serine or LPS) into phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine or PS) (LPSAT activity). Has also weak lysophosphatidylethanolamine acyltransferase activity (LPEAT activity). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs. Seems to be the major enzyme contributing to LPCAT activity in the liver. Lysophospholipid acyltransferases (LPLATs) catalyze the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle.