Rat Metastasis Associated Protein 2 (MTA2) ELISA Kit | MTA2 elisa kit

Rat Metastasis Associated Protein 2 (MTA2) ELISA Kit

Cat. Num. AAA2705866

• Gene Names: MTA2; PID; MTA1L1
• Reactivity: Rat
• Synonyms: Metastasis Associated Protein 2 (MTA2); Rat Metastasis Associated Protein 2 (MTA2) ELISA Kit; MTA1L1; PID; Metastasis-associated 1-like 1; p53 target protein in deacetylase complex; Metastasis-associated protein MTA2; MTA2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Metastasis Associated Protein 2 (MTA2).; No significant cross-reactivity or interference between Metastasis Associated Protein 2 (MTA2) and analogues was observed.
• Sequence Length: 495

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Metastasis Associated Protein 2 (MTA2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Metastasis Associated Protein 2 (MTA2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTA2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metastasis Associated Protein 2 (MTA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metastasis Associated Protein 2 (MTA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metastasis Associated Protein 2 (MTA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Metastasis Associated Protein 2 (MTA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14141170
• NCBI GeneID: 9219
• NCBI Accession #: NP_004730.2
• NCBI GenBank Nucleotide #: NM_004739.3
• UniProt Accession #: O94776; Q68DB1; Q9UQB5
• Molecular Weight: 55,037 Da
• NCBI Official Full Name: metastasis-associated protein MTA2 isoform 1
• NCBI Official Synonym Full Names: metastasis associated 1 family member 2
• NCBI Official Symbol: MTA2
• NCBI Official Synonym Symbols: PID; MTA1L1
• NCBI Protein Information: metastasis-associated protein MTA2
• UniProt Protein Name: Metastasis-associated protein MTA2
• Protein Family: Metastasis-associated protein
• UniProt Gene Name: MTA2
• UniProt Synonym Gene Names: MTA1L1; PID; MTA1-L1 protein

NCBI Description

This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis. [provided by RefSeq, May 2011]

Uniprot Description

May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.