Testing Data
(Peptide-+)
GIT1 Antibody | anti-GIT1 antibody
GIT1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
GIT1; GIT1 Antibody; ARF GTPase-activating protein GIT1; G protein-coupled receptor kinase-interactor 1; similar to G protein-coupled receptor kinase-interactor 1; G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein; anti-GIT1 antibody
Reactivity
Human, Mouse, Rat
Specificity
GIT1 antibody detects endogenous levels of total GIT1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
770
Applicable Applications for anti-GIT1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
IHC: 1:50~1:100
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized peptide derived from C-terminal of human GIT1.
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(Peptide-+)
Testing Data
(Peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using GIT1 antibody. Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using GIT1 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using GIT1 antibody. Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using GIT1 antibody.)
References
Premont R.T., J. Biol. Chem. 275:22373-22380(2000). The MGC Project Team, Genome Res. 14:2121-2127(2004). Premont R.T., Proc. Natl. Acad. Sci. U.S.A. 95:14082-14087(1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,446 Da
NCBI Official Full Name
ARF GTPase-activating protein GIT1 isoform 1
NCBI Official Synonym Full Names
GIT ArfGAP 1
NCBI Official Symbol
GIT1
NCBI Protein Information
ARF GTPase-activating protein GIT1
UniProt Protein Name
ARF GTPase-activating protein GIT1
Protein Family
UniProt Gene Name
GIT1
UniProt Synonym Gene Names
ARF GAP GIT1; CAT-1; CAT1
Uniprot Description
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 ().
Research Articles on GIT1
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Product Notes
The GIT1 git1 (Catalog #AAA8509801) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The GIT1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GIT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000. Researchers should empirically determine the suitability of the GIT1 git1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GIT1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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