Mouse Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) ELISA Kit | MUSK elisa kit

Mouse Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) ELISA Kit

Cat. Num. AAA2705435

• Reactivity: Mouse
• Synonyms: Muscle; Skeletal; Receptor Tyrosine Kinase (MUSK); Mouse Muscle; Receptor Tyrosine Kinase (MUSK) ELISA Kit; Muscle-specific tyrosine-protein kinase receptor; Muscle-specific kinase receptor; MUSK elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK).; No significant cross-reactivity or interference between Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) and analogues was observed.
• Sequence Length: 878

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUSK elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 394933752
• UniProt Accession #: Q61006; Q61005; Q61987; Q61988
• Molecular Weight: 98,081 Da
• NCBI Official Full Name: muscle, skeletal, receptor tyrosine kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muscle, skeletal receptor tyrosine protein kinase
• UniProt Gene Name: Musk
• UniProt Synonym Gene Names: Nsk2; MuSK; Muscle-specific kinase receptor

Uniprot Description

Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.