Human Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) ELISA Kit | MUSK elisa kit

Human Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) ELISA Kit

Cat. Num. AAA2019846

• Gene Names: Musk; Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• Reactivity: Human
• Synonyms: Muscle; Skeletal; Receptor Tyrosine Kinase (MUSK); Human Muscle; Receptor Tyrosine Kinase (MUSK) ELISA Kit; Muscle-specific tyrosine-protein kinase receptor; Muscle-specific kinase receptor; MUSK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK).; No significant cross-reactivity or interference between Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) and analogues was observed.
• Sequence Length: 860

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUSK elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MUSK elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 394933752
• NCBI GeneID: 18198
• UniProt Accession #: Q61006; Q61005; Q61987; Q61988
• Molecular Weight: 98,081 Da
• NCBI Official Full Name: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: Musk
• NCBI Official Synonym Symbols: Mlk; Mdk4; Nsk1; Nsk2; Nsk3
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muscle, skeletal receptor tyrosine protein kinase
• UniProt Gene Name: Musk
• UniProt Synonym Gene Names: Nsk2; MuSK; Muscle-specific kinase receptor
• UniProt Entry Name: MUSK_MOUSE

NCBI Description

This gene encodes a member of the protein tyrosine kinase family. The encoded protein is a type 1 receptor-like protein located in muscle membrane that is activated by the heparan sulfate proteoglycan agrin released by nerve cells. The encoded protein activates signaling cascades responsible for multiple aspects of motor neuron and muscle development, including organization of the postsynaptic membrane, synaptic gene transcription, patterning of skeletal muscle, anchoring of acetylcholinesterase, and guidance of motor axons. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Kinase, protein; TK group; Musk family

Cellular Component: postsynaptic membrane; cell projection; membrane; integral to plasma membrane; plasma membrane; integral to membrane; synapse; cell junction; neuromuscular junction; receptor complex; external side of plasma membrane

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; protein kinase binding; ATP binding; protein kinase activity; PDZ domain binding

Biological Process: positive regulation of synaptic transmission, cholinergic; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of synaptic growth at neuromuscular junction; signal transduction; receptor clustering; protein amino acid phosphorylation; memory; positive regulation of peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; regulation of synaptic growth at neuromuscular junction; positive regulation of protein amino acid phosphorylation; response to electrical stimulus; cell differentiation; phosphorylation; neuromuscular junction development