Rat Adrenergic Receptor Alpha 2C (ADRa2C) ELISA Kit | ADRa2C elisa kit

Rat Adrenergic Receptor Alpha 2C (ADRa2C) ELISA Kit

Cat. Num. AAA2705325

• Gene Names: ADRA2C; ADRA2L2; ADRARL2; ADRA2RL2; ALPHA2CAR
• Reactivity: Rat
• Synonyms: Adrenergic Receptor Alpha 2C (ADRa2C); Rat Adrenergic Receptor Alpha 2C (ADRa2C) ELISA Kit; ADRA2L2; ADRA2RL2; ADRARL2; ALPHA2CAR; Alpha-2 adrenergic receptor subtype C4; Alpha-2C adrenoreceptor; ADRa2C elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Alpha 2C (ADRa2C).; No significant cross-reactivity or interference between Adrenergic Receptor Alpha 2C (ADRa2C) and analogues was observed.
• Sequence Length: 462

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.122ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenergic Receptor Alpha 2C (ADRa2C) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenergic Receptor Alpha 2C (ADRa2C) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADRa2C elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Alpha 2C (ADRa2C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Alpha 2C (ADRa2C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Alpha 2C (ADRa2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Alpha 2C (ADRa2C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71773208
• NCBI GeneID: 152
• NCBI Accession #: NP_000674.2
• NCBI GenBank Nucleotide #: NM_000683.3
• UniProt Accession #: P18825; P35369; Q9HB49
• Molecular Weight: 49,522 Da
• NCBI Official Full Name: alpha-2C adrenergic receptor
• NCBI Official Synonym Full Names: adrenoceptor alpha 2C
• NCBI Official Symbol: ADRA2C
• NCBI Official Synonym Symbols: ADRA2L2; ADRARL2; ADRA2RL2; ALPHA2CAR
• NCBI Protein Information: alpha-2C adrenergic receptor
• UniProt Protein Name: Alpha-2C adrenergic receptor
• Protein Family: Alpha-2C adrenergic receptor
• UniProt Gene Name: ADRA2C
• UniProt Synonym Gene Names: ADRA2L2; ADRA2RL2; Alpha-2C adrenoceptor; Alpha-2CAR

NCBI Description

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]

Uniprot Description

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.