Human Dermatopontin (DPT) ELISA Kit | DPT elisa kit

Human Dermatopontin (DPT) ELISA Kit

Cat. Num. AAA2704448

• Gene Names: DPT; TRAMP
• Reactivity: Human
• Synonyms: Dermatopontin (DPT); Human Dermatopontin (DPT) ELISA Kit; TRAMP; Tyrosine-rich acidic matrix protein; DPT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dermatopontin (DPT).; No significant cross-reactivity or interference between Dermatopontin (DPT) and analogues was observed.
• Sequence Length: 201

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dermatopontin (DPT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dermatopontin (DPT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dermatopontin (DPT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dermatopontin (DPT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dermatopontin (DPT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dermatopontin (DPT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32307172
• NCBI GeneID: 1805
• NCBI Accession #: NP_001928.2
• NCBI GenBank Nucleotide #: NM_001937.4
• UniProt Accession #: Q07507; Q8N4R2; Q9UIX8; A8K981
• Molecular Weight: 24,005 Da
• NCBI Official Full Name: dermatopontin
• NCBI Official Synonym Full Names: dermatopontin
• NCBI Official Symbol: DPT
• NCBI Official Synonym Symbols: TRAMP
• NCBI Protein Information: dermatopontin
• UniProt Protein Name: Dermatopontin
• Protein Family: Dermatopontin
• UniProt Gene Name: DPT
• UniProt Synonym Gene Names: TRAMP

NCBI Description

Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. [provided by RefSeq, Jul 2008]

Uniprot Description

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation ().