Mouse Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit | PPARd elisa kit

Mouse Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit

Cat. Num. AAA2704105

• Gene Names: PPARD; FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB
• Reactivity: Mouse
• Synonyms: Peroxisome Proliferator Activated Receptor Delta (PPARd); Mouse Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit; FAAR; NR1C2; NUC1; NUCI; PPARB; PPARd/B; Nuclear Receptor Subfamily 1; Group C; Member 2; Nuclear hormone receptor 1; Peroxisome proliferator-activated receptor beta; PPARd elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Delta (PPARd).; No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Delta (PPARd) and analogues was observed.
• Sequence Length: 343

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Delta (PPARd) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Delta (PPARd) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPARd elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Delta (PPARd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Delta (PPARd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Delta (PPARd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Delta (PPARd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 60115375
• NCBI GeneID: 5467
• UniProt Accession #: Q03181; Q5D1P0; Q7Z5K0; Q9BUD4; A8K6J6; B4E3V3; B6ZGS1; B7Z3W1; E9PE18
• Molecular Weight: 38,855 Da
• NCBI Official Full Name: peroxisome proliferator activated receptor delta
• NCBI Official Synonym Full Names: peroxisome proliferator activated receptor delta
• NCBI Official Symbol: PPARD
• NCBI Official Synonym Symbols: FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB
• NCBI Protein Information: peroxisome proliferator-activated receptor delta
• UniProt Protein Name: Peroxisome proliferator-activated receptor delta
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: PPARD
• UniProt Synonym Gene Names: NR1C2; PPARB; PPAR-delta; NUC1; PPAR-beta

NCBI Description

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. The encoded protein is thought to function as an integrator of transcriptional repression and nuclear receptor signaling. It may inhibit the ligand-induced transcriptional activity of peroxisome proliferator activated receptors alpha and gamma, though evidence for this effect is inconsistent. Expression of this gene in colorectal cancer cells may be variable but is typically relatively low. Knockout studies in mice suggested a role for this protein in myelination of the corpus callosum, lipid metabolism, differentiation, and epidermal cell proliferation. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2017]

Uniprot Description

Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.