Rat Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit | MIP3b elisa kit

Rat Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit

Cat. Num. AAA2701162

• Gene Names: CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19
• Reactivity: Rat
• Synonyms: Macrophage Inflammatory Protein 3 Beta (MIP3b); Rat Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit; CCL19; CKb11; ELC; MIP3-B; SCYA19; Chemokine C-C-Motif Ligand 19; Beta Chemokine Exodus-3; CK Beta-11; EBI1-Ligand Chemokine; Small Inducible Cytokine Subfamily A19; MIP3b elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 3 Beta (MIP3b).; No significant cross-reactivity or interference between Macrophage Inflammatory Protein 3 Beta (MIP3b) and analogues was observed.
• Sequence Length: 98

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Beta (MIP3b) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Beta (MIP3b) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP3b elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Beta (MIP3b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Beta (MIP3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Beta (MIP3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Beta (MIP3b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1791003
• NCBI GeneID: 6363
• Molecular Weight: 10,993 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 beta
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 19
• NCBI Official Symbol: CCL19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; MIP-3b; SCYA19
• NCBI Protein Information: C-C motif chemokine 19
• UniProt Protein Name: C-C motif chemokine 19
• UniProt Gene Name: CCL19
• UniProt Synonym Gene Names: ELC; MIP3B; SCYA19; EBI1 ligand chemokine; ELC; MIP-3-beta

NCBI Description

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]

Uniprot Description

May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.