Mouse Aquaporin 8 (AQP-8) ELISA Kit | AQP-8 elisa kit

Mouse Aquaporin 8 (AQP-8) ELISA Kit

Cat. Num. AAA262151

• Gene Names: AQP8; AQP-8
• Reactivity: Mouse
• Synonyms: Aquaporin 8 (AQP-8); Mouse Aquaporin 8 (AQP-8) ELISA Kit; AQP-8 elisa kit

• Reactivity: Mouse
• Sequence Length: 261

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-8 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse AQP-8 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 6606076
• NCBI GeneID: 343
• Molecular Weight: 27,381 Da
• NCBI Official Full Name: aquaporin 8
• NCBI Official Synonym Full Names: aquaporin 8
• NCBI Official Symbol: AQP8
• NCBI Official Synonym Symbols: AQP-8
• NCBI Protein Information: aquaporin-8
• UniProt Protein Name: Aquaporin-8
• UniProt Gene Name: AQP8
• UniProt Synonym Gene Names: AQP-8
• UniProt Entry Name: AQP8_HUMAN

NCBI Description

Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP8: Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter; Transporter, aquaporin family; Channel, misc.; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: intracellular canaliculus; intracellular membrane-bound organelle; integral to plasma membrane; apical part of cell; plasma membrane

Molecular Function: water channel activity; glycerol channel activity

Biological Process: glycerol transport; response to reactive oxygen species; cellular water homeostasis; transport; canalicular bile acid transport; water transport; transmembrane transport