Rat Insulin Like Growth Factor Binding Protein 1 (IGFBP-1) ELISA Kit | IGFBP-1 elisa kit

Rat Insulin Like Growth Factor Binding Protein 1 (IGFBP-1) ELISA Kit

Cat. Num. AAA262062

• Gene Names: IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• Reactivity: Rat
• Synonyms: Insulin Like Growth Factor Binding Protein 1 (IGFBP-1); Rat Insulin Like Growth Factor Binding Protein 1 (IGFBP-1) ELISA Kit; IGFBP-1 elisa kit

• Reactivity: Rat
• Sequence Length: 259

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IGFBP-1 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat IGFBP-1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504615
• NCBI GeneID: 3484
• NCBI Accession #: NP_000587.1
• NCBI GenBank Nucleotide #: NM_000596.2
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 1
• NCBI Official Symbol: IGFBP1
• NCBI Official Synonym Symbols: AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• NCBI Protein Information: insulin-like growth factor-binding protein 1; IBP-1; IGFBP-1; binding protein-25; binding protein-26; binding protein-28; placental protein 12; IGF-binding protein 1; amniotic fluid binding protein; growth hormone independent-binding protein; alpha-pregnancy-associated endometrial globulin
• UniProt Protein Name: Insulin-like growth factor-binding protein 1
• UniProt Gene Name: IGFBP1
• UniProt Synonym Gene Names: IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12
• UniProt Entry Name: IBP1_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of insulin-like growth factor receptor signaling pathway; tissue regeneration; unfolded protein response; insulin receptor signaling pathway; signal transduction; positive regulation of cell growth