Sheep IGFBP-1 ELISA Kit | IGFBP-1 elisa kit

Sheep IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit

Cat. Num. AAA2505450

• Gene Names: IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• Reactivity: Sheep
• Synonyms: IGFBP-1; Sheep IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit; IGFBP-1 elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep IGFBP-1. No significant cross-reactivity or interference between Sheep IGFBP-1 and analogues was observed.
• Sequence Length: 259

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP-1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep IGFBP-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IGFBP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IGFBP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IGFBP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IGFBP-1. You can calculate the concentration of IGFBP-1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504615
• NCBI GeneID: 3484
• NCBI Accession #: NP_000587.1
• NCBI GenBank Nucleotide #: NM_000596.2
• UniProt Accession #: P08833; Q8IYP5; A4D2F4; D3DVL9
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 1
• NCBI Official Symbol: IGFBP1
• NCBI Official Synonym Symbols: AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• NCBI Protein Information: insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; placental protein 12
• UniProt Protein Name: Insulin-like growth factor-binding protein 1
• UniProt Gene Name: IGFBP1
• UniProt Synonym Gene Names: IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12
• UniProt Entry Name: IBP1_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: regulation of insulin-like growth factor receptor signaling pathway; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; tissue regeneration; unfolded protein response; insulin receptor signaling pathway; signal transduction; positive regulation of cell growth