Human Chemerin (CHEM) ELISA Kit | CHEM elisa kit

Human Chemerin (CHEM) ELISA Kit

Cat. Num. AAA262025

• Gene Names: CMKLR1; DEZ; RVER1; ChemR23; CHEMERINR
• Reactivity: Human
• Synonyms: Chemerin (CHEM); Human Chemerin (CHEM) ELISA Kit; CHEM elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 373

• Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Ch50 Concentration
• Assay Type: Sandwich
• Detection Range: 100 U/ml-1.56 U/ml
• Sensitivity: 0.5 U/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHEM elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Human CH50 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 215272321
• NCBI GeneID: 1240
• NCBI Accession #: NP_001135817.1
• NCBI GenBank Nucleotide #: NM_001142345.1
• Molecular Weight: 42,035 Da
• NCBI Official Full Name: chemokine-like receptor 1 isoform a
• NCBI Official Synonym Full Names: chemokine-like receptor 1
• NCBI Official Symbol: CMKLR1
• NCBI Official Synonym Symbols: DEZ; RVER1; ChemR23; CHEMERINR
• NCBI Protein Information: chemokine-like receptor 1; chemerin receptor; resolvin E1 receptor; chemokine receptor-like 1; G-protein coupled receptor DEZ; G-protein coupled receptor ChemR23; orphan G-protein coupled receptor, Dez
• UniProt Protein Name: Chemokine-like receptor 1
• UniProt Gene Name: CMKLR1
• UniProt Synonym Gene Names: CHEMR23; DEZ
• UniProt Entry Name: CML1_HUMAN

Uniprot Description

CMKLR1: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF- kappa-B. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2). Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; chemokine receptor activity; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of interleukin-12 production; inhibition of NF-kappaB transcription factor; regulation of calcium-mediated signaling; positive regulation of fat cell differentiation; immune response; chemotaxis; skeletal development