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Plasma Sample Diluent

Plasma Sample Diluent (Antigen-Down SD)

Synonyms
Plasma Sample Diluent; Plasma Sample Diluent (Antigen-Down SD); Plasma Sample Diluent diluent
Ordering
For Research Use Only!
Form/Format
Supplied at 1X.
Application Notes
When diluting test samples, first note the dilution factor for future data analysis. For example, a human plasma sample was diluted 1:10 (50 uL plasma sample + 450 uL plasma sample diluent) and yielded a standard curve derived analyte concentration of 50 ng/mL from the ELISA assay. This concentration should then be multiplied by 10 (the dilution factor) to obtain the actual concentration value of 500 ng/mL in the undiluted plasma sample.
pH
7.4 at 1X
Contains
Mammalian Proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Plasma Sample Diluent diluent
Description: Plasma Sample Diluent is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This novel mammalian-protein-buffered formulation provides an assay environment that minimizes non-specific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. Plasma Sample Diluent also inhibits thrombin activity (clotting) and complement activity during the plasma sample incubation period.

Background: Plasma Sample Diluent should be utilized as a buffer matrix for diluting out plasma samples in antigen-down or antigen-capture ELISA formats. This diluent is also an excellent choice for diluting out antigen standards in ELISA standard curve preparation. This ELISA sample diluent contains proprietary additives in a buffered BSA protein base providing an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction.

Specially formulated additives inhibit thrombin activity (clotting) and complement activity during the plasma sample incubation period. Plasma Sample Diluent also contains antimicrobial agents to allow for room temperature benchtop use and extensive storage stability.
Product Categories/Family for Plasma Sample Diluent diluent

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Product Notes

The Plasma Sample Diluent (Catalog #AAA258404) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. When diluting test samples, first note the dilution factor for future data analysis. For example, a human plasma sample was diluted 1:10 (50 uL plasma sample + 450 uL plasma sample diluent) and yielded a standard curve derived analyte concentration of 50 ng/mL from the ELISA assay. This concentration should then be multiplied by 10 (the dilution factor) to obtain the actual concentration value of 500 ng/mL in the undiluted plasma sample. Researchers should empirically determine the suitability of the Plasma Sample Diluent for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Plasma Sample Diluent, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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