Neptune Block ELISA Blocking Buffer
Neptune Block ELISA Blocking Buffer
Synonyms
Neptune Block ELISA Blocking Buffer; Neptune Block ELISA Blocking Buffer blocking buffer
Form/Format
Supplied at 1X.
Application Notes
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
pH
7.4 at 1X
Contains
Non-Mammalian Proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Neptune Block ELISA Blocking Buffer blocking buffer
Description: Neptune Block Blocking Buffer is non-mammalian and designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. This buffer is particularly useful when working with human and other mammalian serum samples as it works to reduce interactions between sample and blocking molecules.
Background: Neptune Block Blocking Buffer is a non-mammalian blocking buffer designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. Neptune Block is particularly useful when working with human and other mammalian serum samples as it works to reduce interactions between sample and blocking molecules.
Neptune Block ELISA Blocking Buffer is a mixture of small molecular proteins and molecular stabilizers capable of blocking the nonspecific binding sites of the adsorbed proteins, as well as the unoccupied regions of the plate that are not accessible to larger mammalian blocking reagents. When used in antigen-down ELISA formats, this product's low molecular-weight proteins reduce the possibility of false-positives generated from endogenous antibodies in the sample reacting with plate-blocking proteins.
This buffer contains an antimicrobial agent for room temperature blocking. Bulk volumes and custom packaging are available upon request.
Background: Neptune Block Blocking Buffer is a non-mammalian blocking buffer designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. Neptune Block is particularly useful when working with human and other mammalian serum samples as it works to reduce interactions between sample and blocking molecules.
Neptune Block ELISA Blocking Buffer is a mixture of small molecular proteins and molecular stabilizers capable of blocking the nonspecific binding sites of the adsorbed proteins, as well as the unoccupied regions of the plate that are not accessible to larger mammalian blocking reagents. When used in antigen-down ELISA formats, this product's low molecular-weight proteins reduce the possibility of false-positives generated from endogenous antibodies in the sample reacting with plate-blocking proteins.
This buffer contains an antimicrobial agent for room temperature blocking. Bulk volumes and custom packaging are available upon request.
Product Categories/Family for Neptune Block ELISA Blocking Buffer blocking buffer
Similar Products
Product Notes
The Neptune Block ELISA Blocking Buffer (Catalog #AAA258384) is a Blocking Buffer and is intended for research purposes only. The product is available for immediate purchase. 1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8-24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT's ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C. Researchers should empirically determine the suitability of the Neptune Block ELISA Blocking Buffer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neptune Block ELISA Blocking Buffer, Blocking Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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