DNMT1 Protein Recombinant Protein | DNMT1 recombinant protein

Recombinant Rat DNMT1 Protein (His Tag)

Cat. Num. AAA2569434

• Purity: >80% as determined by reducing SDS-PAGE
• Synonyms: DNMT1 Protein; Recombinant Rat DNMT1 Protein (His Tag); DNA (cytosine-5)-methyltransferase 1; Dnmt1; Met-1; DNA methyltransferase MmuI; DNA MTase MmuI; M.MmuI; MCMT; Dnmt; Met1; Uim; DNMT1 recombinant protein

• Host: E Coli
• Purity/Purification: >80% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4
• Sequence: Phe950-Pro1142

• Species: Rat
• Tag: N-His
• Protein Construction: A DNA sequence encoding the Rat DNMT1 (Q9Z330-1)(Phe950-Pro1142) was expressed with a polyhistidine tag at the N-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for DNMT1 recombinant protein

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.

NCBI and Uniprot Product Information

• NCBI GI #: 20137608
• NCBI GeneID: 84350
• UniProt Accession #: Q9Z330; P70487; Q9R252; Q9WTX3; Q9WU57
• Molecular Weight: 169,645 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 1
• NCBI Official Synonym Full Names: DNA methyltransferase 1
• NCBI Official Symbol: Dnmt1
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 1
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 1
• UniProt Gene Name: Dnmt1
• UniProt Synonym Gene Names: Dnmt1; M.RnoIP

NCBI Description

methylates genomic DNA; involved in processes of gene inactivation, chromatin organization, X chromosome inactivation and genomic imprinting [RGD, Feb 2006]

Uniprot Description

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: Amino Acid Metabolism - cysteine and methionine; Cell development/differentiation; EC 2.1.1.37; Methyltransferase; Methyltransferase, DNA; Transcription regulation

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: cell soma; centric heterochromatin; cytoplasm; heterochromatin; nucleus; protein complex; replication fork

Molecular Function: chromatin binding; DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; estrogen receptor binding; histone deacetylase binding; methyl-CpG binding; methyltransferase activity; protein binding; protein domain specific binding; RNA binding; S-adenosylmethionine-dependent methyltransferase activity; zinc ion binding

Biological Process: aging; brain development; covalent chromatin modification; cytosine methylation within a CG sequence; DNA methylation; DNA methylation involved in embryo development; DNA methylation on cytosine; gene silencing; maintenance of DNA methylation; negative regulation of histone H3-K9 methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron differentiation; positive regulation of histone H3-K4 methylation; Ras protein signal transduction; regulation of cell proliferation; regulation of gene expression; response to activity; response to caffeine; response to drug; response to estradiol; response to ethanol; response to heat; response to ionizing radiation; response to lead ion; response to lipopolysaccharide; response to nutrient levels; response to organic substance; response to testosterone stimulus; response to toxin; response to vitamin A; S-adenosylmethionine metabolic process; transcription, DNA-dependent