Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded Human tonsil tissue with Epithelial Membrane Antigen(EMA) Monoclonal Antibody)
Mouse anti-Human Epithelial Membrane Antigen (EMA) Monoclonal Antibody
Epithelial Membrane Antigen (EMA) Monoclonal Antibody
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Epithelial Membrane Antigen (EMA); Monoclonal Antibody; Epithelial Membrane Antigen (EMA) Monoclonal Antibody; anti-Epithelial Membrane Antigen (EMA) antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
YN00796m
Applicable Applications for anti-Epithelial Membrane Antigen (EMA) antibody
Immunohistochemistry (IHC)
Application Notes
This product is used for immunohistochemical staining of formalin fixed paraffin embedded or frozen sections. The recommended dilution of concentrated product is 1:50-1:200, and RTU is ready to use. Repair the antigens in pH9.0 EDTA solution by microwave or high-pressure boiling before incubating the antibodies. This product is the primary antibody required for immunohistochemistry experiment, which needs to be followed with secondary antibody, DAB kit, hematoxylin staining solution and other reagents. This product is suitable for the immunohistochemistry kits produced by various manufacturers.
Localization
Cell membrane, Cytoplasm
Incubation
Incubate at RT (18-30 degree C) for 60 min or incubate at 4 degree C for overnight.
Preparation and Storage
The concentrated antibody should be stored at -20 degree C protecting from light. It is recommended to sub-pack into several small tubes for the first use. Take one tube each time. Avoid freeze / thaw cycles. The RTU antibody should be stored at 2~8 degree C protecting from light (Do not freeze). Please restore at 2~8 degree C immediately after each time use. The expiration of this antibody is 12 months. Please follow this manual to store the antibody to avoid badly affecting the titer and effective service life of the product.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded Human tonsil tissue with Epithelial Membrane Antigen(EMA) Monoclonal Antibody)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffinembedded Human tonsil tissue with Epithelial Membrane Antigen(EMA) Monoclonal Antibody)
Related Product Information for anti-Epithelial Membrane Antigen (EMA) antibody
Epithelial Membrane Antigen (EMA) is a group of glycoprotein, which is widely expressed in various normal epithelial cell membranes and tumors. The expression range is similar to that of cytokeratin, but its expression in visceral gland epithelium is better than that of cytokeratin. Therefore, for epithelial tumors, especially for poorly differentiated adenocarcinoma, it is better to combine with cytokeratin to improve the positive rate. EMA high temperature repair can enhance staining strength.
Similar Products
Product Notes
The Epithelial Membrane Antigen (EMA) (Catalog #AAA2567654) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Epithelial Membrane Antigen (EMA) Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Epithelial Membrane Antigen (EMA) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). This product is used for immunohistochemical staining of formalin fixed paraffin embedded or frozen sections. The recommended dilution of concentrated product is 1:50-1:200, and RTU is ready to use. Repair the antigens in pH9.0 EDTA solution by microwave or high-pressure boiling before incubating the antibodies. This product is the primary antibody required for immunohistochemistry experiment, which needs to be followed with secondary antibody, DAB kit, hematoxylin staining solution and other reagents. This product is suitable for the immunohistochemistry kits produced by various manufacturers. Researchers should empirically determine the suitability of the Epithelial Membrane Antigen (EMA) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Epithelial Membrane Antigen (EMA), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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