Rabbit anti-Human, Mouse PDLIM5 Polyclonal Antibody | anti-PDLIM5 antibody

PDLIM5 Polyclonal Antibody

Cat. Num. AAA3011770

• Gene Names: PDLIM5; L9; ENH; LIM; ENH1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PDLIM5; Polyclonal Antibody; PDLIM5 Polyclonal Antibody; PDZ and LIM domain protein 5; Enigma homolog; Enigma-like PDZ and LIM domains protein; ENH; L9; anti-PDLIM5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PDLIM5 polyclonal antibody detects endogenous levels of PDLIM5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 596

• Applicable Applications for anti-PDLIM5 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human PDLIM5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using PDLIM5 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using PDLIM5 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PDLIM5 antibody

PDLIM5 (PDZ and LIM domain 5), also known as L9, ENH (Enigma homolog), LIM or ENH1, is a member of the Enigma family of proteins. Proteins belonging to this family contain an N-terminal PDZ (post-synaptic density-95/discs large/zone occludens-1) domain and one to three C-terminal LIM domains that typically associate with various isoforms of PKC (protein kinase C). Expressed in a wide variety of tissues, PDLIM5 contains three LIM zinc-binding domains and one PDZ domain. In the brain, PDLIM5 colocalizes with synaptic vesicles of neurotransmitters and regulates neuronal calcium signaling through an interaction with PKC epsilon and N-type Ca++ CP alpha1B (N-type calcium channel alpha1B subunit). Expression of PDLIM5 is often increased in the brain of patients with schizophrenia, major depression and bipolar disorder. This suggests that PDLIM5 may contribute to the genetic susceptibility of such conditions.

NCBI and Uniprot Product Information

• NCBI GI #: 317373590
• NCBI GeneID: 10611
• UniProt Accession #: Q96HC4
• Molecular Weight: ~ 63kDa
• NCBI Official Full Name: PDZ and LIM domain protein 5
• NCBI Official Synonym Full Names: PDZ and LIM domain 5
• NCBI Official Symbol: PDLIM5
• NCBI Official Synonym Symbols: L9; ENH; LIM; ENH1
• NCBI Protein Information: PDZ and LIM domain protein 5
• UniProt Protein Name: PDZ and LIM domain protein 5
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM5
• UniProt Synonym Gene Names: ENH
• UniProt Entry Name: PDLI5_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

LIM: an adaptor protein of the Enigma family. Contains three LIM domains and one PDZ domain. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. Enigma family proteins possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: postsynaptic membrane; neuron projection; membrane; postsynaptic density; cell junction; Z disc; cytosol; actin cytoskeleton

Molecular Function: actinin binding; protein kinase C binding; zinc ion binding; protein N-terminus binding; actin binding

Biological Process: regulation of synaptogenesis