CD157/BST1 recombinant protein

Recombinant Human CD157/BST1 Protein (C-His)

Cat. Num. AAA2557384

• Gene Names: BST1; CD157
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: CD157/BST1; Recombinant Human CD157/BST1 Protein (C-His); ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2; ADP-ribosyl cyclase 2; Bone marrow stromal antigen 1; BST-1; Cyclic ADP-ribose hydrolase 2; cADPr hydrolase 2; CD157; CD157/BST1 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Gly29-Lys292
• Sequence Length: 318

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for CD157/BST1 recombinant protein

Protein Construction: Recombinant Human BST1 is produced by our mammalian expression system and the target gene encoding Gly29-Lys292 is expressed with a 6His tag at the C-terminus.

Background: The cluster of differentiation (CD) system is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family. It is generally used in immunophynotyping. CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth. CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities followed with CD38. It plays a role in rheumatoid arthritis (RA) due to its enhanced expression in RA-derived bone marrow stromal cell lines. Studies have shown that this protein have a role in predicted to function as a cell surface receptor and an immunoregulatory molecule.

NCBI and Uniprot Product Information

• NCBI GI #: 168229159
• NCBI GeneID: 683
• NCBI Accession #: NP_004325.2
• NCBI GenBank Nucleotide #: NP_004325.2
• UniProt Accession #: Q10588
• Molecular Weight: Molecular Mass: 30.8kDa; Actual Protein Molecular Mass: 37kDa
• NCBI Official Full Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
• NCBI Official Synonym Full Names: bone marrow stromal cell antigen 1
• NCBI Official Symbol: BST1
• NCBI Official Synonym Symbols: CD157
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
• UniProt Protein Name: ADP-ribosyl cyclase 2
• UniProt Gene Name: BST1
• UniProt Synonym Gene Names: BST-1
• UniProt Entry Name: BST1_HUMAN

NCBI Description

Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq, Jul 2008]

Uniprot Description

BST1: Synthesizes cyclic ADP-ribose, a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. Belongs to the ADP-ribosyl cyclase family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Hydrolase; EC 3.2.2.6; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: metabolic process; multicellular organismal development; positive regulation of B cell proliferation; humoral immune response