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SDS-Page (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

CD157 recombinant protein

Recombinant CD157

Gene Names
BST1; CD157
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
CD157; Recombinant CD157; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2; BST1; CD157 recombinant protein
Ordering
For Research Use Only!
Host
Baculovirus
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.5mg/ml (determined by Absorbance at 280nm.) (varies by lot)
Sequence Positions
33-293aa
Sequence
RWRGEGTSAH LRDIFLGRCA EYRALLSPEQ RNKNCTAIWE AFKVALDKDP CSVLPSDYDL FINLSRHSIP RDKSLFWENS HLLVNSFADN TRRFMPLSDV LYGRVADFLS WCRQKNDSGL DYQSCPTSED CENNPVDSFW KRASIQYSKD SSGVIHVMLN GSEPTGAYPI KGFFADYEIP NLQKEKITRI EIWVMHEIGG PNVESCGEGS MKVLEKRLKD MGFQYSCIND YRPVKLLQCV DHSTHPDCAL KSAAAATQRK AHHHHHH
Sequence Length
318
Applicable Applications for CD157 recombinant protein
SDS-PAGE
Species
Human
Endotoxin
<0.1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Arg33
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

SDS-Page (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for CD157 recombinant protein
BST1, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, is a glycosylphosphatidyl inositol (GPI) anchored membrane protein that belongs to the CD38 family. It was originally identified as a bone marrow stromal cell molecule. This protein is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. Along with CD38, it exhibits both DP-ribosyl cyclase and cyclinc ADP ribose hydrolase activities. It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA-derived bone marrow stromal cell lines. Also, it is expressed by cells of the myeloid lineage and could act as a receptor with signal transduction capability. Recombinant human BST1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CD157 recombinant protein
References
Kaisho T., et al, (1994) Proc. Natl. Acad. Sci. U.S.A. 91:5325-5329. Okuyama Y., et al, (1996) Biochem. Biophys. Res. Commun. 228:838-845.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
683
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30.5kDa (267aa); 28-40kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
NCBI Official Synonym Full Names
bone marrow stromal cell antigen 1
NCBI Official Symbol
BST1
NCBI Official Synonym Symbols
CD157
NCBI Protein Information
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
UniProt Protein Name
ADP-ribosyl cyclase 2
UniProt Gene Name
BST1
UniProt Synonym Gene Names
BST-1
UniProt Entry Name
BST1_HUMAN

NCBI Description

Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq, Jul 2008]

Uniprot Description

BST1: Synthesizes cyclic ADP-ribose, a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. Belongs to the ADP-ribosyl cyclase family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Hydrolase; EC 3.2.2.6; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: metabolic process; multicellular organismal development; positive regulation of B cell proliferation; humoral immune response

Research Articles on CD157

Similar Products

Product Notes

The CD157 bst1 (Catalog #AAA201067) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 33-293aa. AAA Biotech's CD157 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CD157 bst1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RWRGEGTSAH LRDIFLGRCA EYRALLSPEQ RNKNCTAIWE AFKVALDKDP CSVLPSDYDL FINLSRHSIP RDKSLFWENS HLLVNSFADN TRRFMPLSDV LYGRVADFLS WCRQKNDSGL DYQSCPTSED CENNPVDSFW KRASIQYSKD SSGVIHVMLN GSEPTGAYPI KGFFADYEIP NLQKEKITRI EIWVMHEIGG PNVESCGEGS MKVLEKRLKD MGFQYSCIND YRPVKLLQCV DHSTHPDCAL KSAAAATQRK AHHHHHH. It is sometimes possible for the material contained within the vial of "CD157, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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