Cathepsin B Active Protein | Ctsb active protein

Recombinant Mouse Cathepsin B Protein

Cat. Num. AAA2556748

• Gene Names: Ctsb; CB
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Cathepsin B; Recombinant Mouse Cathepsin B Protein; Ctsb; Cathepsin B1; Ctsb active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 339

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Met 1-Phe 339
• Fusion Tag: C-6His
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The specific activity is >2,000 pmoles/min/mug.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for Ctsb active protein

Protein Construction: Recombinant Mouse Cathepsin B is produced by our Mammalian expression system and the target gene encoding His18-Phe339 is expressed with a 6His tag at the C-terminus.

Background: Cathepsin B (CatB) is an enzymatic protein belonging to the peptidase (or protease) families. It is the first described member of the family of lysosomal cysteine proteases. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI). It is believed to participate in intracellular degradation and turnover of proteins. Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Cathepsin B may also have a role in invasion and metastasis.

NCBI and Uniprot Product Information

• NCBI GI #: 6681079
• NCBI GeneID: 13030
• NCBI Accession #: NP_031824.1
• NCBI GenBank Nucleotide #: NM_007798.3
• UniProt Accession #: P10605; Q3UDW7
• Molecular Weight: Molecular Mass: 36.4kDa; Actual Protein Molecular Mass: 32-50kDa
• NCBI Official Full Name: cathepsin B preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: Ctsb
• NCBI Official Synonym Symbols: CB
• NCBI Protein Information: cathepsin B
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsb

NCBI Description

This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Has also been implicated in tumor invasion and metastasis.