Rabbit anti-Mouse Cathepsin B Monoclonal Antibody | anti-CTSB antibody

Recombinant Anti-Cathepsin B Antibody, Rabbit Monoclonal

Cat. Num. AAA8110935

• Gene Names: Ctsb; CB
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: Cathepsin B; Monoclonal Antibody; Recombinant Anti-Cathepsin B Antibody; Rabbit Monoclonal; Cathepsin B/CTSB Antibody; Rabbit MAb; cathepsin B; Anti-CB Antibody; anti-CTSB antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 010
• Specificity: Mouse Cathepsin B/CTSB
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CTSB antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-1:500
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse Cathepsin B/CTSB Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Cathepsin B/CTSB (rM Cathepsin B/CTSB).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CTSB in mouse liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CTSB in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-CTSB antibody

Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

References

Mai J, et al. (2000) Cell surface complex of cathepsin B/annexin II tetramer in malignant progression. Biochim Biophys Acta. 1477(1-2): 215-30.Podgorski I, et al. (2003) Cathepsin B and its role(s) in cancer progression. Biochem Soc Symp. (70): 263-76.Yan S, et al. (2003) Molecular regulation of human cathepsin B: implication in pathologies. Biol Chem. 384(6): 845-54.Roshy S, et al. (2003) Pericellular cathepsin B and malignant progression. Cancer Metastasis Rev. 22(2-3): 271-86.

NCBI and Uniprot Product Information

• NCBI GI #: 6681079
• NCBI GeneID: 13030
• NCBI Accession #: NP_031824.1
• NCBI GenBank Nucleotide #: NM_007798.3
• UniProt Accession #: P10605; Q3UDW7
• Molecular Weight: 37,280 Da
• NCBI Official Full Name: cathepsin B preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: Ctsb
• NCBI Official Synonym Symbols: CB
• NCBI Protein Information: cathepsin B
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsb

NCBI Description

This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Autophagy; EC 3.4.22.1; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 14 D1|14 33.24 cM

Cellular Component: apical plasma membrane; caveola; cell surface; cytoplasm; external side of plasma membrane; extracellular region; extracellular space; intracellular membrane-bound organelle; lysosome; mitochondrion; nucleolus; perinuclear region of cytoplasm; sarcolemma

Molecular Function: collagen binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; kininogen binding; peptidase activity; peptide binding; protein complex binding; protein self-association; proteoglycan binding

Biological Process: collagen catabolic process; decidualization; entry of virus into host cell; proteolysis; proteolysis involved in cellular protein catabolic process