Jagged-1 Active Protein | JAG1 active protein

Recombinant Human Jagged-1 Protein

Cat. Num. AAA2556661

• Gene Names: JAG1; AGS; AHD; AWS; HJ1; AGS1; DCHE; CD339; JAGL1
• Purity: >90% as determined by reducing SDS-PAGE.
• Synonyms: Jagged-1; Recombinant Human Jagged-1 Protein; Protein jagged-1 I; JAGL1; HJ1; JAG1 and CD339; JAG1 active protein

• Host: Human Cells
• Purity/Purification: >90% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Length: 1218

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fragment: Gln34-Ser1046
• Fusion Tag: C-Fc
• Activity: Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells.The ED50 for this effect is typically 4-20 mug/mL.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

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Related Product Information for JAG1 active protein

Protein Construction: Recombinant Human jagged-1 is produced by our Mammalian expression system and the target gene encoding Gln34-Ser1046 is expressed with a Fc tag at the C-terminus.

Background: Protein jagged-1 I, also known as Jagged-1, JAGL1, HJ1, JAG1 and CD339, is a single-pass type I membrane protein. JAG1 contains one DSL domain and sixteen EGF-like domain. JAG1 acts as a ligand for multiple Notch receptors and is involved in the mediation of Notch signaling. JAG1 may participate in early and late stages of mammalian cardiovascular development, JAG1 inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis. Defects in JAG1 are the cause of Alagille syndrome type 1, which is autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations.

NCBI and Uniprot Product Information

• NCBI GI #: 4557679
• NCBI GeneID: 182
• NCBI Accession #: NP_000205.1
• NCBI GenBank Nucleotide #: NM_000214.3
• UniProt Accession #: P78504; O14902; O15122; Q15816; A0AV43; B4DYR1; E9PCF9
• Molecular Weight: Molecular Mass: 138.5kDa; Actual Protein Molecular Mass: 141kDa
• NCBI Official Full Name: protein jagged-1
• NCBI Official Synonym Full Names: jagged canonical Notch ligand 1
• NCBI Official Symbol: JAG1
• NCBI Official Synonym Symbols: AGS; AHD; AWS; HJ1; AGS1; DCHE; CD339; JAGL1
• NCBI Protein Information: protein jagged-1
• UniProt Protein Name: Protein jagged-1
• Protein Family: Protein jagged
• UniProt Gene Name: JAG1
• UniProt Synonym Gene Names: JAGL1; Jagged1; hJ1

NCBI Description

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed:18660822, PubMed:20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed:9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (). Enhances fibroblast growth factor-induced angiogenesis (in vitro).