Mouse anti-Human Jagged 1 Monoclonal Antibody | anti-JAG1 antibody

Anti-Jagged 1 Antibody (PE), Mouse Monoclonal

Cat. Num. AAA8105566

• Gene Names: JAG1; AGS; AHD; AWS; HJ1; AGS1; DCHE; CD339; JAGL1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: Jagged 1; Monoclonal Antibody; Anti-Jagged 1 Antibody (PE); Mouse Monoclonal; JAG1/Jagged 1/CD339 Antibody (PE); Mouse MAb; jagged 1; Anti-AGS Antibody; Anti-AHD Antibody; Anti-AWS Antibody; Anti-CD339 Antibody; Anti-HJ1 Antibody; Anti-Jagged 1 Antibody; Anti-JAGL1 Antibody; anti-JAG1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 03
• Specificity: Human JAG1/Jagged 1
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-JAG1 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human JAG1/Jagged 1 protein
• Conjugation: PE
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human JAG1/Jagged 1 (rh JAG1/Jagged 1) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Profile of anti-JAG1(CD339) reactivity on HepG2 cells analyzed by flow cytometry.)

Related Product Information for anti-JAG1 antibody

Protein Jagged 1, also known as JAG1, JAGL1, and CD339, is a single-pass type I membrane protein that contains 1 DSL domain and 15 EGF-like domains. JAG1/Jagged 1 is widely expressed in adult and fetal tissues. The expression of JAG1/Jagged 1 is up-regulated in cervical squamous cell carcinoma. JAG1/Jagged 1 is also expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. JAG1/Jagged 1 is a ligand for multiple Notch receptors. It is involved in the mediation of Notch signaling. JAG1/Jagged 1 may be involved in cell-fate decisions during hematopoiesis. JAG1/Jagged 1 seems to be involved in the early and late stages of mammalian cardiovascular development. It inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis. Defects in JAG1/Jagged 1 are the cause of Alagille syndrome type 1 (ALGS1). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. Defects in JAG1/Jagged 1 are also a cause of tetralogy of Fallot (TOF). TOF is a congenital heart anomaly that consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left), and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation.

Product Categories/Family for anti-JAG1 antibody

Notch Pathway

References

Oda T.et al., 1997, Nat. Genet. 16:235-242.Krantz I.D. et al., 1998, Am. J. Hum. Genet. 62:1361-1369.Li L. et al., 1998, Immunity. 8:43-55.Jones E.A. et al., 2000, J. Med. Genet. 37: 658-662.Roepke A.et al., 2003, Hum. Mutat. 21:100-100.Jurkiewicz D.et al., 2005, Hum. Mutat. 25:321-321.Warthen D.M.et al., 2006, Hum. Mutat. 27:436-443.

NCBI and Uniprot Product Information

• NCBI GI #: 4557679
• NCBI GeneID: 182
• NCBI Accession #: NP_000205.1
• NCBI GenBank Nucleotide #: NM_000214.3
• UniProt Accession #: P78504; O14902; O15122; Q15816; A0AV43; B4DYR1; E9PCF9
• Molecular Weight: 116,579 Da
• NCBI Official Full Name: protein jagged-1
• NCBI Official Synonym Full Names: jagged 1
• NCBI Official Symbol: JAG1
• NCBI Official Synonym Symbols: AGS; AHD; AWS; HJ1; AGS1; DCHE; CD339; JAGL1
• NCBI Protein Information: protein jagged-1
• UniProt Protein Name: Protein jagged-1
• Protein Family: Protein jagged
• UniProt Gene Name: JAG1
• UniProt Synonym Gene Names: JAGL1; Jagged1; hJ1
• UniProt Entry Name: JAG1_HUMAN

NCBI Description

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008]

Uniprot Description

JAG1: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Interacts with NOTCH2 and NOTCH3. Interacts with NOTCH1 (in the presence of calcium ions). Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12.1-p11.23

Cellular Component: membrane; apical part of cell; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; calcium ion binding; structural molecule activity; Notch binding

Biological Process: endothelial cell differentiation; nervous system development; Notch signaling pathway; positive regulation of myeloid cell differentiation; T cell mediated immunity; Notch receptor processing; auditory receptor cell differentiation; cell fate determination; negative regulation of fat cell differentiation; regulation of cell migration; regulation of cell proliferation; keratinocyte differentiation; myoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; response to muramyl dipeptide; morphogenesis of an epithelial sheet; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; blood vessel remodeling; angiogenesis; positive regulation of Notch signaling pathway

Disease: Tetralogy Of Fallot; Alagille Syndrome 1