Western Blot (WB)
((0.3ug/ml) staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Goat Plakoglobin/Gamma-catenin Polyclonal Antibody | anti-JUP antibody
Goat anti-Plakoglobin/Gamma-catenin Antibody
Western Blot: Approx 80kDa band observed in lysates of cell lines A431A 549 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3ug/ml.
Enzyme immunoassay: Customer finds it successful in quantitative ELISA assays (EIA) but the antibody is very sensitive to freeze/thaw cycles in this assay.
Western Blot (WB)
((0.3ug/ml) staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((0.3ug/ml) staining of A431 (A) and NIH3T3 (B) cell lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
(Staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of Rat Eye lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]
Uniprot Description
CTNNG: Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Homodimer. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma- catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1. Interacts with PTPRJ. Interacts with DSC2. Belongs to the beta-catenin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: actin cytoskeleton; apicolateral plasma membrane; catenin complex; cell-cell adherens junction; cytoplasm; cytoskeleton; cytosol; desmosome; fascia adherens; focal adhesion; hemidesmosome; intercellular junction; intermediate filament; internal side of plasma membrane; lateral plasma membrane; nucleus; plasma membrane; Z disc; zonula adherens
Molecular Function: alpha-catenin binding; cadherin binding; cell adhesion molecule binding; protein binding; protein homodimerization activity; protein kinase binding; protein phosphatase binding; signal transducer activity; structural constituent of cell wall; structural molecule activity; transcription coactivator activity
Biological Process: cell migration; cell-cell adhesion; cytoskeletal anchoring; detection of mechanical stimulus; positive regulation of protein import into nucleus; positive regulation of transcription factor activity; protein heterooligomerization; regulation of cell proliferation; signal transduction; skin development
Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 12; Naxos Disease
Research Articles on JUP
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Product Notes
The JUP jup (Catalog #AAA420515) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Plakoglobin/Gamma-catenin Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, and may cross-react with other species as described in the data sheet. AAA Biotech's Plakoglobin/Gamma-catenin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 80kDa band observed in lysates of cell lines A431A 549 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3ug/ml. Enzyme immunoassay: Customer finds it successful in quantitative ELISA assays (EIA) but the antibody is very sensitive to freeze/thaw cycles in this assay. Researchers should empirically determine the suitability of the JUP jup for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GLRPPYPTAD HMLA. It is sometimes possible for the material contained within the vial of "Plakoglobin/Gamma-catenin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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