SLAM Family Member 5/SLAMF5/CD84 Recombinant Protein | SLAMF5/CD84 recombinant protein

Recombinant Human SLAM Family Member 5/SLAMF5/CD84 Protein (C-6His)

Cat. Num. AAA2553238

• Gene Names: CD84; LY9B; hCD84; mCD84; SLAMF5
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: SLAM Family Member 5/SLAMF5/CD84; Recombinant Human SLAM Family Member 5/SLAMF5/CD84 Protein (C-6His); SLAM family member 5; Cell surface antigen MAX.3; Hly9-beta; Leukocyte differentiation antigen CD84; Signaling lymphocytic activation molecule 5; CD84; SLAMF5; SLAMF5/CD84 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Positions: Lys22-Arg220
• Sequence Length: 345

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for SLAMF5/CD84 recombinant protein

SLAM family member 5 (SLAMF5/CD84) is a type I transmembrane protein in the SLAM subgroup of the CD2 family. SLAM family proteins regulate multiple aspects of immune system function. Mature human CD84 consists of a 204 amino acid (aa) extracellular domain (ECD) with two Iglike domains, a 21 aa transmembrane segment, and a 99 aa cytoplasmic domain with two immunoreceptor tyrosinebased switch motifs (ITSMs). CD84 exhibits homophilic binding which is mediated by the N-terminal Ig-like domain. Ligation induces tyrosine phosphorylation in the cytoplasmic ITSMs which then recruit the signaling adaptor molecules SAP (SLAM-associated protein) and EAT-2(EWS/Fli1-activated transcript 2).CD84 signaling inhibits Fc epsilon RI-induced mast cell activation but enhances platelet activation. LPS-induced macrophage activation, T cell proliferation and IFN-gammaproduction, and the interactions between T cells and B cells that are required for germinal center formation.

NCBI and Uniprot Product Information

• NCBI GI #: 296434285
• NCBI GeneID: 8832
• NCBI Accession #: NP_001171808.1
• NCBI GenBank Nucleotide #: NM_001184879.1
• UniProt Accession #: Q9UIB8; O15430; O95266; O95660; Q5H9R1; Q6FHA8; Q8WLP1; Q8WWI8; Q9UF04; Q9UIB6; B2R8T1; B7Z3R8
• Molecular Weight: Molecular Mass: 23.1 kDa; Actual Protein Molecular Mass: 35-43 kDa
• NCBI Official Full Name: SLAM family member 5 isoform 1
• NCBI Official Synonym Full Names: CD84 molecule
• NCBI Official Symbol: CD84
• NCBI Official Synonym Symbols: LY9B; hCD84; mCD84; SLAMF5
• NCBI Protein Information: SLAM family member 5
• UniProt Protein Name: SLAM family member 5
• UniProt Gene Name: CD84
• UniProt Synonym Gene Names: SLAMF5

NCBI Description

This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]

Uniprot Description

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (). Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells (PubMed:11564780, PubMed:12115647. PubMed:12928397, PubMed:12962726, PubMed:16037392) Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity (). In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1 (PubMed:22068234). In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2 (). Positively regulates macroautophagy in primary dendritic cells via stabilization of IRF8; inhibits TRIM21-mediated proteasomal degradation of IRF8 (PubMed:29434592).