Rabbit anti-Human PPP1R3G Polyclonal Antibody | anti-PPP1R3G antibody

PPP1R3G Antibody (C-term)

Cat. Num. AAA288181

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: PPP1R3G, Antibody; PPP1R3G Antibody (C-term); Protein phosphatase 1 regulatory subunit 3G; PPP1R3G; anti-PPP1R3G antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PPP1R3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-298 amino acids from the C-terminal region of human PPP1R3G.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 269-298
• Sequence Length: 358

• Applicable Applications for anti-PPP1R3G antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: Glycogen-targeting subunit for protein phosphatase 1 (PP1). Involved in the regulation of hepatic glycogenesis in a manner coupled to the fasting-feeding cycle and distinct from other glycogen-targeting subunits (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(PPP1R3G Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(All lanes : Anti-PPP1R3G Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane2: Jurkat whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: U-87 MG whole cell lysate Lane 5: HepG2 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(PPP1R3G Antibody (C-term) western blot analysis in K562,Jurkat cell line lysates (35ug/lane).This demonstrates the PPP1R3G antibody detected the PPP1R3G protein (arrow).)

Product Categories/Family for anti-PPP1R3G antibody

Metabolism; Signal Transduction

References

Ceulemans, H., et al. Bioessays 24(4):371-381(2002)

NCBI and Uniprot Product Information

• NCBI GI #: 223278393
• NCBI GeneID: 648791
• NCBI Accession #: NP_001138587.1
• NCBI GenBank Nucleotide #: NM_001145115.2
• UniProt Accession #: B7ZBB8
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 3G
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 3G
• NCBI Official Symbol: PPP1R3G
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 3G
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 3G
• UniProt Gene Name: PPP1R3G
• UniProt Entry Name: PP13G_HUMAN