Autophagy Related 3 Homolog/ATG3 Recombinant Protein | ATG3 recombinant protein

Recombinant Human Autophagy Related 3 Homolog/ATG3 Protein (C-6His, N-T7 tag)

Cat. Num. AAA2552377

• Gene Names: ATG3; APG3; APG3L; PC3-96; APG3-LIKE
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Autophagy Related 3 Homolog/ATG3; Recombinant Human Autophagy Related 3 Homolog/ATG3 Protein (C-6His; N-T7 tag); Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; ATG3; APG3; APG3L; ATG3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 20mM Tris HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
• Sequence Positions: Met1-Met314
• Sequence Length: 311

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Store at < -20 degree C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Related Product Information for ATG3 recombinant protein

Ubiquitin-like-conjugating enzyme ATG3 (ATG3), also known as Apg3L and Apg3p, functions as a regulatory component of autophagosome biogenesis necessary for autophagy. ATG3 exhibits 98% aa sequence identity with both its mouse and rat orthologs. It is widely expressed and has highly levels in heart, skeletal muscle, kidney, liver and placenta. As an E2-like enzyme, involves in autophagy and mitochondrial homeostasis. ATG3 catalyzes the conjugation of ATG8-like proteins to PE which is essential for autophagy. ATG3 also can catalyze the conjugation of ATG12 to itself which palys a role in mitochondrial homeostasis but not in autophagy.

NCBI and Uniprot Product Information

• NCBI GI #: 523704487
• NCBI GeneID: 64422
• NCBI Accession #: NP_001265641.1
• NCBI GenBank Nucleotide #: NM_001278712.1
• UniProt Accession #: Q9NT62; Q6PKC5; Q9H6L9
• Molecular Weight: Molecular Mass: 36 kDa; Actual Protein Molecular Mass: 38 kDa
• NCBI Official Full Name: ubiquitin-like-conjugating enzyme ATG3 isoform 2
• NCBI Official Synonym Full Names: autophagy related 3
• NCBI Official Symbol: ATG3
• NCBI Official Synonym Symbols: APG3; APG3L; PC3-96; APG3-LIKE
• NCBI Protein Information: ubiquitin-like-conjugating enzyme ATG3
• UniProt Protein Name: Ubiquitin-like-conjugating enzyme ATG3
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG3
• UniProt Synonym Gene Names: APG3; APG3L; APG3-like; hApg3

NCBI Description

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.