Axl Kinase Recombinant Protein | Axl recombinant protein

Recombinant Mouse Axl Kinase Protein (His & Fc tag)

Cat. Num. AAA2547148

• Gene Names: Axl; Ark; Ufo; Tyro7; AI323647
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: Axl Kinase; Recombinant Mouse Axl Kinase Protein (His & Fc tag); AI323647; Ark; Tyro7; Ufo; Axl recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% mannitol, 0.01% Tween-80

• Source: Mouse
• Predicted N Terminal: His 20
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Pro 443) of mouse AXL (NP_033491.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Molecular Mass: The recombinant mouse AXL/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 672 amino acids and has a predicted molecular mass of 74.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmAXL/Fc monomer is approximately 100-110 kDa due to glycosylation.
• Reconstitution: It is recommended that sterile water (400uL) be added to vial to prepare a stock solution of 0.25 mg/ml.; Concentration is measured by UV-Vis
• Preparation and Storage: Storage: Store under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.; Stability: Samples are stable for up to 12 months from date of receipt at -70°C.

SDS-PAGE

Related Product Information for Axl recombinant protein

Axl receptor tyrosine kinase, together with Tyro3 and Mer, constitute the TAM family of receptor tyrosine kinases. In the nervous system, Axl and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell type s. Axl functions in dampening the immune response, regulating cytokine secretion, clearing apoptotic cells and debris, and maintaining cell survival. Axl is upregulated in various disease states, such as in the cuprizone toxicity-induced model of demyelination and in multiple sclerosis (MS) lesions, suggesting that it plays a role in disease pathogenesis. Axl expression correlates with poor prognosis in several cancers. Axl mediates multiple oncogenic phenotype s and activation of these RTKs constitutes a mechanism of chemoresistance in a variety of solid tumors. Axl contributes to cell survival, migration, invasion, metastasis and chemosensitivity justify further investigation of Axl as novel therapeutic targets in cancer. The receptor tyrosine kinase AXL is thought to play a role in metastasis. The soluble AXL receptor as a therapeutic candidate agent for treatment of metastatic ovarian cancer. GAS6/AXL targeting as an effective strategy for inhibition of metastatic tumor progression in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 31542164
• NCBI GeneID: 26362
• NCBI Accession #: NP_033491.2
• NCBI GenBank Nucleotide #: NM_009465.4
• UniProt Accession #: Q00993; Q80YQ3
• Molecular Weight: 98,191 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor UFO isoform 1
• NCBI Official Synonym Full Names: AXL receptor tyrosine kinase
• NCBI Official Symbol: Axl
• NCBI Official Synonym Symbols: Ark; Ufo; Tyro7; AI323647
• NCBI Protein Information: tyrosine-protein kinase receptor UFO
• UniProt Protein Name: Tyrosine-protein kinase receptor UFO
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: Axl
• UniProt Synonym Gene Names: Ark; Ufo

Uniprot Description

AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Axl family; EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 7 A3|7 14.02 cM

Cellular Component: cell surface; extracellular space

Molecular Function: myosin heavy chain binding; phosphatidylserine binding; phosphoinositide 3-kinase binding; protein heterodimerization activity; protein-tyrosine kinase activity

Biological Process: apoptotic cell clearance; blood vessel remodeling; cell differentiation; cellular response to extracellular stimulus; entry of virus into host cell; enzyme linked receptor protein signaling pathway; erythrocyte homeostasis; forebrain cell migration; inflammatory response; natural killer cell differentiation; negative regulation of apoptosis; negative regulation of cytokine production; negative regulation of interferon-gamma production; negative regulation of lymphocyte activation; negative regulation of neuron apoptosis; negative regulation of tumor necrosis factor production; neuron migration; ovulation cycle; phagocytosis; platelet activation; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of natural killer cell differentiation; positive regulation of pinocytosis; positive regulation of protein kinase B signaling cascade; protein kinase B signaling cascade; secretion by cell; spermatogenesis; vagina development