CD16 / FCGR3 recombinant protein

Recombinant Mouse CD16 / FCGR3 Protein (His tag)

Cat. Num. AAA2547032

• Gene Names: Fcgr3; CD16
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD16 / FCGR3; Recombinant Mouse CD16 / FCGR3 Protein (His tag); CD16; Fcgr3; CD16 / FCGR3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 261

• Application Notes: The secreted recombinant mouse FCGR3 consists of 196 amino acids and has a predicted molecular mass of 22.6 kDa. As a result of glycosylation, the apparent molecular mass of rmFCGR3 is approximately 35-40 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 31
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD16 / FCGR3 recombinant protein

Background: Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcgammaR). FcgammaR is divided into three classes, Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcgammaRIIIa and CD16b/FcgammaRIIIb) encoded by two different highly homologous genes in a cell type -specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

Description: A DNA sequence encoding the mouse FCGR3 (P08508-1) extracellular domain (Met 1-Thr 215) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 119877
• NCBI GeneID: 14131
• UniProt Accession #: P08508
• Molecular Weight: 30,036 Da
• NCBI Official Full Name: Low affinity immunoglobulin gamma Fc region receptor III
• NCBI Official Synonym Full Names: Fc receptor, IgG, low affinity III
• NCBI Official Symbol: Fcgr3
• NCBI Official Synonym Symbols: CD16
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III
• UniProt Gene Name: Fcgr3
• UniProt Synonym Gene Names: IgG Fc receptor III; FcRIII

Uniprot Description

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1 H3|1 78.8 cM

Cellular Component: external side of plasma membrane

Molecular Function: IgG binding; IgG receptor activity

Biological Process: antibody-dependent cellular cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell surface receptor linked signal transduction; mast cell activation; neutrophil chemotaxis; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of type I hypersensitivity; positive regulation of type IIa hypersensitivity; positive regulation of type III hypersensitivity; regulation of immune response; serotonin secretion