Rabbit anti-Mouse CD16/FCGR3 Monoclonal Antibody | anti-FCGR3 antibody

Recombinant Anti-CD16/FCGR3 Antibody (PE), Rabbit Monoclonal

Cat. Num. AAA8111393

• Gene Names: Fcgr3; CD16
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD16/FCGR3; Monoclonal Antibody; Recombinant Anti-CD16/FCGR3 Antibody (PE); Rabbit Monoclonal; FCGR3 Antibody (PE); Rabbit MAb; Fc receptor; IgG; low affinity III; Anti-CD16 Antibody; anti-FCGR3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 002
• Specificity: Mouse CD16/FCGR3
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-FCGR3 antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse CD16/FCGR3 protein
• Conjugation: PE
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD16/FCGR3 (rM CD16/FCGR3) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Mouse FCGR3(CD16) expression on mouse splenocytes. BABL/c splenocytes were stained with PE-conjugated anti-Mouse FCGR3(CD16) and FITC-conjugated anti-Mouse CD3 (BD Pharmingen'). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-FCGR3 antibody

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcgammaR). FcgammaR is divided into three classes, Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcgammaRIIIa and CD16b/FcgammaRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

Product Categories/Family for anti-FCGR3 antibody

Fc Receptors

References

Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.

NCBI and Uniprot Product Information

• NCBI GI #: 119877
• NCBI GeneID: 14131
• UniProt Accession #: P08508
• NCBI Official Full Name: Low affinity immunoglobulin gamma Fc region receptor III
• NCBI Official Synonym Full Names: Fc receptor, IgG, low affinity III
• NCBI Official Symbol: Fcgr3
• NCBI Official Synonym Symbols: CD16
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: Fcgr3
• UniProt Synonym Gene Names: IgG Fc receptor III; FcRIII
• UniProt Entry Name: FCGR3_MOUSE

Uniprot Description

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Membrane protein, integral; Cell surface; Receptor, misc.

Cellular Component: external side of plasma membrane; integral to membrane; membrane; plasma membrane

Molecular Function: IgG binding; IgG receptor activity

Biological Process: antibody-dependent cellular cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I; cell surface receptor linked signal transduction; mast cell activation; neutrophil chemotaxis; phagocytosis, engulfment; phagocytosis, recognition; positive regulation of phagocytosis; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of type I hypersensitivity; positive regulation of type IIa hypersensitivity; positive regulation of type III hypersensitivity; regulation of immune response; serotonin secretion