Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD16 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD16, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Mouse anti-Human CD16/FCGR3 Monoclonal Antibody | anti-FCGR3 antibody
Anti-CD16/FCGR3 Antibody, Mouse Monoclonal
Gene Names
FCGR3B; CD16; FCG3; CD16A; CD16b; FCGR3; FCGR3A; FCR-10; FCRIII; FCRIIIb
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD16/FCGR3; Monoclonal Antibody; Anti-CD16/FCGR3 Antibody; Mouse Monoclonal; CD16/Fc gamma RIII Antibody; Mouse MAb; Fc receptor; IgG; low affinity III; Anti-CD16 Antibody; Anti-Fc gamma RIII Antibody; Anti-FCRIII Antibody; anti-FCGR3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
10
Specificity
Human CD16 (CD16a & CD16b)//FCGR3
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FCGR3 antibody
Flow Cytometry (FC/FACS/FCM)
Application Notes
FC/FACS/FCM: 1:25-1:100
Immunogen
Recombinant Human CD16b/Fc gamma RIIIb Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16b/Fc gamma RIIIb (rh CD16b/Fc gamma RIIIb). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD16 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD16, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD16 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD16, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Related Product Information for anti-FCGR3 antibody
Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcgammaR). FcgammaR is divided into three classes, Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcgammaRIIIa and CD16b/FcgammaRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Product Categories/Family for anti-FCGR3 antibody
References
Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
26,216 Da
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor III-B
NCBI Official Synonym Full Names
Fc fragment of IgG receptor IIIb
NCBI Official Symbol
FCGR3B
NCBI Official Synonym Symbols
CD16; FCG3; CD16A; CD16b; FCGR3; FCGR3A; FCR-10; FCRIII; FCRIIIb
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-B
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-B
Protein Family
UniProt Gene Name
FCGR3B
UniProt Synonym Gene Names
CD16B; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb
NCBI Description
The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1. [provided by RefSeq, Aug 2012]
Uniprot Description
Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Research Articles on FCGR3
Similar Products
Product Notes
The FCGR3 fcgr3b (Catalog #AAA8104678) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD16/FCGR3 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD16/FCGR3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the FCGR3 fcgr3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16/FCGR3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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