beta-NGF / Beta-NGF Recombinant Protein | beta-NGF recombinant protein

Recombinant Mouse beta-NGF / Beta-NGF Protein

Cat. Num. AAA2547009

• Gene Names: Ngf; Ngfb
• Purity: > 96 % as determined by SDS-PAGE
• Synonyms: beta-NGF / Beta-NGF; Recombinant Mouse beta-NGF / Beta-NGF Protein; Ngf; Ngfb; beta-NGF recombinant protein

• Host: CHO Stable Cells
• Purity/Purification: > 96 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5
• Sequence Length: 241

• Application Notes: The recombinant mouse NGF consists of 121 amino acids and has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for beta-NGF recombinant protein

Background: Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, alpha, beta, and gamma, among which, the beta subunit, called beta-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Description: A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 162951831
• NCBI GeneID: 18049
• NCBI Accession #: NP_001106168.1
• NCBI GenBank Nucleotide #: NM_001112698.2
• UniProt Accession #: P01139; Q63864; Q6LDB7
• Molecular Weight: 27,077 Da
• NCBI Official Full Name: beta-nerve growth factor isoform B
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3 F2.2|3 45.25 cM

Cellular Component: cytoplasmic vesicle; extracellular region; extracellular space

Molecular Function: growth factor activity; metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; receptor signaling protein activity; transmembrane receptor protein tyrosine kinase activator activity

Biological Process: adult locomotory behavior; circadian rhythm; induction of apoptosis via death domain receptors; memory; negative regulation of neuron apoptosis; neurite development; neurite morphogenesis; neuron apoptosis; peripheral nervous system development; positive regulation of axon extension; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of collateral sprouting; positive regulation of nerve growth factor receptor signaling pathway; positive regulation of neuron differentiation; positive regulation of neuron maturation; positive regulation of protein amino acid autophosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of protein ubiquitination; positive regulation of Ras protein signal transduction; positive regulation of transcription factor activity; regulation of neuron differentiation; regulation of neurotransmitter secretion; regulation of release of sequestered calcium ion into cytosol; sensory perception of pain