NGF recombinant protein

NGF Protein, Mouse, Recombinant

Cat. Num. AAA8118842

• Gene Names: Ngf; Ngfb
• Purity: >96 % as determined by SDS-PAGE
• Synonyms: NGF; NGF Protein; Mouse; Recombinant; Mouse beta-NGF/Beta-NGF Protein; nerve growth factor (beta polypeptide); Ngfb Protein; NGF recombinant protein

• Host: CHO Stable Cells
• Purity/Purification: >96 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH5.5 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 121aa
• Sequence: Ser122-Gly241

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Protein Construction: |A DNA sequence encoding the mature form of mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed, with an initial Met at the C-terminus
• Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-8ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-8ng/mL.)

Related Product Information for NGF recombinant protein

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, alpha, beta, and gamma, among which, the beta subunit, called beta-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Product Categories/Family for NGF recombinant protein

Death Receptor Signaling

References

Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.

NCBI and Uniprot Product Information

• NCBI GI #: 162951831
• NCBI GeneID: 18049
• NCBI Accession #: NP_001106168.1
• NCBI GenBank Nucleotide #: NM_001112698.2
• UniProt Accession #: P01139; Q63864; Q6LDB7
• Molecular Weight: 27,077 Da
• NCBI Official Full Name: beta-nerve growth factor isoform B
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb
• NCBI Protein Information: beta-nerve growth factor; beta-NGF; nerve growth factor, beta
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF
• UniProt Entry Name: NGF_MOUSE

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasmic membrane-bound vesicle; extracellular region

Molecular Function: metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity; receptor binding

Biological Process: positive regulation of neuron maturation; adult locomotory behavior; regulation of neuron differentiation; positive regulation of axon extension; regulation of neurotransmitter secretion; sensory perception of pain; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; regulation of release of sequestered calcium ion into cytosol; memory; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; positive regulation of cell proliferation; positive regulation of transcription factor activity; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; positive regulation of collateral sprouting; transmembrane receptor protein tyrosine kinase signaling pathway; neurite development; neurite morphogenesis