SDS-PAGE
ENPP7 / NPP-7 recombinant protein
Recombinant Mouse ENPP7 / NPP-7 Protein (His tag)
Gene Names
Enpp7; Gm254; Alk-SMase
Purity
> 90 % as determined by SDS-PAGE
Synonyms
ENPP7 / NPP-7; Recombinant Mouse ENPP7 / NPP-7 Protein (His tag); RP23-386D6.4; Alk-SMase; Gm254; ENPP7 / NPP-7 recombinant protein
Host
Human Cells
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.6
Sequence Length
439
Application Notes
The secreted recombinant mouse ENPP7 consists of 411 amino acids and has a calculated molecular mass of 47 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
Predicted N Terminal
Ala 22
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for ENPP7 / NPP-7 recombinant protein
Background: Mouse Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
Description: A DNA sequence encoding the mouse ENPP7 (Q3TIW9) (Met 1-Gln 421) was expressed, with a polyhistidine tag at the C-terminus.
Description: A DNA sequence encoding the mouse ENPP7 (Q3TIW9) (Met 1-Gln 421) was expressed, with a polyhistidine tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,618 Da
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 7 isoform X1
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 7
NCBI Official Symbol
Enpp7
NCBI Official Synonym Symbols
Gm254; Alk-SMase
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 7
UniProt Protein Name
Enpp7 protein
UniProt Gene Name
Enpp7
Uniprot Description
ENPP7: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Protein type: Cell cycle regulation; DNA replication; EC 3.1.4.12; Lipid Metabolism - sphingolipid; Membrane protein, integral; Motility/polarity/chemotaxis; Phosphodiesterase
Chromosomal Location of Human Ortholog: 11|11 E2
Cellular Component: Golgi apparatus; microvillus
Molecular Function: nucleotidase activity; sphingomyelin phosphodiesterase activity
Biological Process: negative regulation of cell proliferation; negative regulation of DNA replication; sphingomyelin catabolic process; sphingomyelin metabolic process
Research Articles on ENPP7 / NPP-7
Similar Products
Product Notes
The ENPP7 / NPP-7 enpp7 (Catalog #AAA2546913) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant mouse ENPP7 consists of 411 amino acids and has a calculated molecular mass of 47 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions due to glycosylation. Researchers should empirically determine the suitability of the ENPP7 / NPP-7 enpp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPP7 / NPP-7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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